ArticleMolecular biology of the cell2000
Specific retrieval of the exocytic SNARE Snc1p from early yeast endosomes.
Article in Molecular biology of the cell, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 194 papers.
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Who cites it
194 citing papers in PubMed, 364 citations in OpenAlex.
- De novo coassembly of functional chlorophyll- and carotenoid-protein complexes inScience advances · 2026Article
- Polarized anionic phospholipids and exocytosis are implicated in the polarized recruitment of budding yeast AP180, an endocytic initiator.Molecular biology of the cell · 2026Article
- Rethinking the Yeast Endomembrane System.Sub-cellular biochemistry · 2026Review
- Endocytosis and trans-Golgi Network in Yeast.Sub-cellular biochemistry · 2026Review
- The actin module of endocytic internalization in Aspergillus nidulans: A critical role of the WISH/DIP/SPIN90 family protein Dip1.PLoS genetics · 2025Article
- Killer toxin K28 resistance in yeast relies on COG complex-mediated trafficking of the defence factor Ktd1.Journal of cell science · 2025Article
- The pathway of unconventional protein secretion involves CUPS and a modified trans-Golgi network.The Journal of cell biology · 2025Article
- Selective clearance of aberrant membrane proteins by TORC1-mediated micro-ER-phagy.Cell reports · 2025Article
- Unremodeled GPI-anchored proteins at the plasma membrane trigger aberrant endocytosis.Life science alliance · 2025Article
- P4-ATPase endosomal recycling relies on multiple retromer-dependent localization signals.Molecular biology of the cell · 2024Article
- Transport mechanisms between the endocytic, recycling, and biosynthetic pathways via endosomes and theFrontiers in cell and developmental biology · 2024Review
- Actin- and microtubule-based motors contribute to clathrin-independent endocytosis in yeast.Molecular biology of the cell · 2023Article
- The SNARE-associated protein Sft2 functions in Imh1-mediated SNARE recycling transport upon ER stress.Molecular biology of the cell · 2023Article
- The yeast endocytic early/sorting compartment exists as an independent sub-compartment within theeLife · 2023Article
- SNARE Protein Snc1 Is Essential for Vesicle Trafficking, Membrane Fusion and Protein Secretion in Fungi.Cells · 2023Review
- Exploring the consequences of redirecting an exocytic Rab onto endocytic vesicles.Molecular biology of the cell · 2023Article
- Design of Nanohydrogels for Targeted Intracellular Drug Transport to the Trans-Golgi Network.Advanced healthcare materials · 2023Article
- The dynamin-related protein Vps1 and the peroxisomal membrane protein Pex27 function together during peroxisome fission.Journal of cell science · 2023Article
- Role of phosphatidylserine in the localization of cell surface membrane proteins in yeast.Cell structure and function · 2023Article
- FgAP1Journal of fungi (Basel, Switzerland) · 2023Article
134 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many endocytosed proteins in yeast travel to the vacuole, but some are recycled to the plasma membrane. We have investigated the recycling of chimeras containing green fluorescent protein (GFP) and the exocytic SNARE Snc1p. GFP-Snc1p moves from the cell surface to internal structures when Golgi function or exocytosis is blocked, suggesting continuous recycling via the Golgi. Internalization is mediated by a conserved cytoplasmic signal, whereas diversion from the vacuolar pathway requires sequences within and adjacent to the transmembrane domain. Delivery from the Golgi to the surface is also influenced by the transmembrane domain, but the requirements are much less specific. Recycling requires the syntaxins Tlg1p and Tlg2p but not Pep12p or proteins such as Vps4p and Vps5p that have been implicated in late endosome-Golgi traffic. Subtle changes to the recycling signal cause GFP-Snc1p to accumulate preferentially in punctate internal structures, although it continues to recycle to the surface. The internal GFP-Snc1p colocalizes with Tlg1p, and immunofluorescence and immunoelectron microscopy reveal structures that contain Tlg1p, Tlg2p, and Kex2p but lack Pep12p and Sec7p. We propose that these represent early endosomes in which sorting of Snc1p and late Golgi proteins occurs, and that transport can occur directly from them to the Golgi apparatus.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.