ArticleThe Journal of experimental medicine2000
In vivo cytotoxic T lymphocyte elicitation by mycobacterial heat shock protein 70 fusion proteins maps to a discrete domain and is CD4(+) T cell independent.
Article in The Journal of experimental medicine, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The trial behind it
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Who cites it
34 citing papers in PubMed, 122 citations in OpenAlex.
- Two homologous sequences of Grp78 and HSP70 represent tumor antigens shared with streptococcal superantigens in eliciting an antitumor immune response: an immunoinformatic investigation.Frontiers in immunology · 2025Article
- Dual roles ofFrontiers in immunology · 2025Article
- Vaccine Potential of a Recombinant Bivalent Fusion Protein LcrV-HSP70 Against Plague and Yersiniosis.Frontiers in immunology · 2020Article
- Plague vaccine: recent progress and prospects.NPJ vaccines · 2019Review
- A Recombinant Trivalent Fusion Protein F1-LcrV-HSP70(II) Augments Humoral and Cellular Immune Responses and Imparts Full Protection against Yersinia pestis.Frontiers in microbiology · 2016Article
- HSP70 domain II of Mycobacterium tuberculosis modulates immune response and protective potential of F1 and LcrV antigens of Yersinia pestis in a mouse model.PLoS neglected tropical diseases · 2014Article
- A novel mycobacterial Hsp70-containing fusion protein targeting mesothelin augments antitumor immunity and prolongs survival in murine models of ovarian cancer and mesothelioma.Journal of hematology & oncology · 2014Article
- VaxCelerate II: rapid development of a self-assembling vaccine for Lassa fever.Human vaccines & immunotherapeutics · 2014Review
- Article
- CD8+ T cells specific for a malaria cytoplasmic antigen form clusters around infected hepatocytes and are protective at the liver stage of infection.Infection and immunity · 2013Article
- Enhanced immunity against hepatoma induced by dendritic cells pulsed with Hsp70-H22 peptide complexes and CD40L.Journal of cancer research and clinical oncology · 2012Article
- Cloning, expression, and homology modeling of GroEL protein from Leptospira interrogans serovar autumnalis strain N2.Genomics, proteomics & bioinformatics · 2011Article
- Vaccination with TCL plus MHSP65 induces anti-lung cancer immunity in mice.Cancer immunology, immunotherapy : CII · 2010Article
- Effect of adjuvants on immune response and protective immunity elicited by recombinant Hsp60 (GroEL) of Salmonella typhi against S. typhi infection.Molecular and cellular biochemistry · 2010Article
- Induction of CD4-independent E7-specific CD8+ memory response by heat shock fusion protein.Clinical and vaccine immunology : CVI · 2007Article
- A reduced antigen load in vivo, rather than weak inflammation, causes a substantial delay in CD8+ T cell priming against Mycobacterium bovis (bacillus Calmette-Guérin).Journal of immunology (Baltimore, Md. : 1950) · 2007Article
- Heat shock proteins HSP70 and GP96: structural insights.Cancer immunology, immunotherapy : CII · 2006Review
- Fusion proteins of Hsp70 with tumor-associated antigen acting as a potent tumor vaccine and the C-terminal peptide-binding domain of Hsp70 being essential in inducing antigen-independent anti-tumor response in vivo.Cell stress & chaperones · 2006Article
- Heat shock protein 70 / MAGE-1 tumor vaccine can enhance the potency of MAGE-1-specific cellular immune responses in vivo.Cancer immunology, immunotherapy : CII · 2004Article
- Cross-presentation: dendritic cells and macrophages bite off more than they can chew!Immunology · 2004Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
To gain insights into the mechanisms by which soluble heat shock protein (hsp) fusions can elicit CD8(+) cytotoxic T lymphocytes (CTLs) against the fusion partner, mycobacterial (Mycobacterium tuberculosis) hsp70 was dissected to ascertain whether a particular hsp domain is necessary, and knockout mice were used to determine whether the fusion protein's immunogenicity is dependent on CD4(+) T lymphocytes. We found that the ability to elicit CD8(+) CTLs depends on a discrete 200-amino acid protein domain, indicating that the fusion protein's immunogenicity for CD8(+) T cells does not require coupled chaperone function or peptide binding. Further, we found that ovalbumin (OVA).hsp70 fusion protein elicited anti-OVA CD8(+) CTLs about equally well in CD4 knockout and wild-type C57BL/6 mice, and also when the hsp70 was of murine (self) origin. The ability of hsp70 fusion proteins to elicit CD4-independent CTL responses suggests that hsp70 fusion proteins may be useful for immunological prophylaxis and therapy against disease in CD4(+) T cell-deficient individuals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.