ArticleThe Journal of experimental medicine2000
A balance between positive and negative signals in cytotoxic lymphocytes regulates the polarization of lipid rafts during the development of cell-mediated killing.
Article in The Journal of experimental medicine, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
37 citing papers in PubMed, 130 citations in OpenAlex.
- The membrane distal domain of CD16a allosterically regulates NK cell ADCC.bioRxiv : the preprint server for biology · 2026Article
- Assessment of membrane lipid state at the natural killer cell immunological synapse.Methods in cell biology · 2023Article
- Degranulation enhances presynaptic membrane packing, which protects NK cells from perforin-mediated autolysis.PLoS biology · 2021Article
- Impact of Plasma Membrane Domains on IgG Fc Receptor Function.Frontiers in immunology · 2020Review
- Evidence for host genetic regulation of altered lipid metabolism in experimental toxoplasmosis supported with gene data mining results.PloS one · 2017Article
- Ras-related C3 Botulinum Toxin Substrate (Rac) and Src Family Kinases (SFK) Are Proximal and Essential for Phosphatidylinositol 3-Kinase (PI3K) Activation in Natural Killer (NK) Cell-mediated Direct Cytotoxicity against Cryptococcus neoformans.The Journal of biological chemistry · 2016Article
- Elastic Membrane Deformations Govern Interleaflet Coupling of Lipid-Ordered Domains.Physical review letters · 2015Article
- L-plastin is involved in NKG2D recruitment into lipid rafts and NKG2D-mediated NK cell migration.Journal of leukocyte biology · 2014Article
- Dynamics of natural killer cell receptor revealed by quantitative analysis of photoswitchable protein.Biophysical journal · 2013Article
- Signaling pathways in aged T cells - a reflection of T cell differentiation, cell senescence and host environment.Seminars in immunology · 2012Review
- Understanding natural killer cell regulation by mathematical approaches.Frontiers in immunology · 2012Article
- Illuminating the dynamics of signal integration in Natural Killer cells.Frontiers in immunology · 2012Article
- Human NKG2D-ligands: cell biology strategies to ensure immune recognition.Frontiers in immunology · 2012Article
- Finding Balance: T cell Regulatory Receptor Expression during Aging.Aging and disease · 2011Article
- The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells.Journal of cell science · 2011Article
- PD-1 expression on peripheral CD8+ TEM/TEMRA subsets closely correlated with HCV viral load in chronic hepatitis C patients.Virology journal · 2010Article
- Significant functional heterogeneity among KIR2DL1 alleles and a pivotal role of arginine 245.Blood · 2009Article
- Endocytosis and intracellular trafficking of human natural killer cell receptors.Traffic (Copenhagen, Denmark) · 2009Review
- Article
- The killer's kiss: the many functions of NK cell immunological synapses.Current opinion in cell biology · 2008Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Plasma membrane microdomains containing sphingolipids and cholesterol (lipid rafts) are enriched in signaling molecules. The cross-linking of certain types of cell surface receptors initiates the redistribution of these lipid rafts, resulting in the formation of signaling complexes. However, little is known about the regulation of the initial raft redistribution and whether negative regulatory signaling pathways target this phase of cellular activation. We used natural killer (NK) cells as a model to investigate the regulation of raft redistribution, as both positive and negative signals have been implicated in the development of their cellular function. Here we show that after NK cells form conjugates with sensitive tumor cells, rafts become polarized to the site of target recognition. This redistribution of lipid rafts requires the activation of both Src and Syk family protein tyrosine kinases. In contrast, engagement of major histocompatibility complex (MHC)-recognizing killer cell inhibitory receptors (KIRs) on NK cells by resistant, MHC-bearing tumor targets blocks raft redistribution. This inhibition is dependent on the catalytic activity of KIR-associated SHP-1, a Src homology 2 (SH2) domain containing tyrosine phosphatase. These results suggest that the influence of integrated positive and negative signals on raft redistribution critically influences the development of cell-mediated cytotoxicity.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.