ArticleThe EMBO journal2000
Identification of an erythroid-enriched endoribonuclease activity involved in specific mRNA cleavage.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 82 citations in OpenAlex.
- A mammalian pre-mRNA 5' end capping quality control mechanism and an unexpected link of capping to pre-mRNA processing.Molecular cell · 2013Article
- Article
- AUF-1 and YB-1 are critical determinants of β-globin mRNA expression in erythroid cells.Blood · 2012Article
- Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.RNA (New York, N.Y.) · 2010Review
- Heterogeneous nuclear ribonucleoproteins (hnRNPs) in cellular processes: Focus on hnRNP E1's multifunctional regulatory roles.RNA (New York, N.Y.) · 2010Review
- Diverse endonucleolytic cleavage sites in the mammalian transcriptome depend upon microRNAs, Drosha, and additional nucleases.Molecular cell · 2010Article
- Identification of Apurinic/apyrimidinic endonuclease 1 (APE1) as the endoribonuclease that cleaves c-myc mRNA.Nucleic acids research · 2009Article
- Functional analysis of a tripartite stability element within the CD40 ligand 3' untranslated region.Immunology · 2008Article
- The 90-kDa heat shock protein stabilizes the polysomal ribonuclease 1 mRNA endonuclease to degradation by the 26S proteasome.Molecular biology of the cell · 2008Article
- Renal response to metabolic acidosis: role of mRNA stabilization.Kidney international · 2008Review
- Phosphorothioate cap analogs stabilize mRNA and increase translational efficiency in mammalian cells.RNA (New York, N.Y.) · 2007Article
- c-Src activates endonuclease-mediated mRNA decay.Molecular cell · 2007Article
- Review
- A nucleolin-binding 3' untranslated region element stabilizes beta-globin mRNA in vivo.Molecular and cellular biology · 2006Article
- Aldolases a and C are ribonucleolytic components of a neuronal complex that regulates the stability of the light-neurofilament mRNA.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Protein synthesis and urokinase mRNA metabolism.Molecular and cellular biochemistry · 2005Article
- Nematode m7GpppG and m3(2,2,7)GpppG decapping: activities in Ascaris embryos and characterization of C. elegans scavenger DcpS.RNA (New York, N.Y.) · 2004Article
- A novel set of nuclear localization signals determine distributions of the alphaCP RNA-binding proteins.Molecular and cellular biology · 2003Article
- An endonuclease activity similar to Xenopus PMR1 catalyzes the degradation of normal and nonsense-containing human beta-globin mRNA in erythroid cells.RNA (New York, N.Y.) · 2003Article
- The KH-domain protein alpha CP has a direct role in mRNA stabilization independent of its cognate binding site.Molecular and cellular biology · 2003Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Stability of the human alpha-globin mRNA is conferred by a ribonucleoprotein complex termed the alpha-complex, which acts by impeding deadenylation. Using our recently devised in vitro decay assay, we demonstrate that the alpha-complex also functions by protecting the 3'-untranslated region (3'-UTR) from an erythroid-enriched, sequence-specific endoribonuclease activity. The cleavage site was mapped to a region protected by the alpha-complex and is regulated by the presence of the alpha-complex. Similar endoribonuclease cleavage products were also detected in erythroid cells expressing an exogenous alpha-globin gene. Nucleotide substitution of the target sequence renders the RNA refractory to the endoribonuclease activity. Insertion of the target sequence onto a heterologous RNA confers sequence-specific cleavage on the chimeric RNA, demonstrating the sequence specificity of this activity. We conclude that the alpha-complex stabilizes the alpha-globin mRNA in erythroid cells by a multifaceted approach, one aspect of which is to protect the 3'-UTR from specific endoribonuclease cleavage.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.