ArticleThe EMBO journal2000
Feedback-regulated degradation of the transcriptional activator Met4 is triggered by the SCF(Met30 )complex.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 76 papers.
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Who cites it
76 citing papers in PubMed, 167 citations in OpenAlex.
- The proportional scaling of mRNA and ribosome concentrations controls eukaryotic cell growth.Nature cell biology · 2026Article
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- Distinct Stress Regulators in the CRL Family: Emerging Roles of F-Box Proteins: Cullin-RING Ligases and Stress-Sensing.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Evidence for a hydrogen sulfide-sensing E3 ligase in yeast.Genetics · 2024Article
- Accurate and sensitive interactome profiling using a quantitative protein-fragment complementation assay.Cell reports methods · 2024Article
- Sulfur starvation-induced autophagy in Saccharomyces cerevisiae involves SAM-dependent signaling and transcription activator Met4.Nature communications · 2024Article
- Cadmium binding by the F-box domain induces p97-mediated SCF complex disassembly to activate stress response programs.Nature communications · 2024Article
- The Swi-Snf chromatin remodeling complex mediates gene repression through metabolic control.Nucleic acids research · 2023Article
- Molecular Biology of Cadmium Toxicity in Saccharomyces cerevisiae.Biological trace element research · 2021Review
- Cdc48/Shp1 participates in dissociation of protein complexes to regulate their activity.Current genetics · 2021Review
- Article
- Cdc48 cofactor Shp1 regulates signal-induced SCFProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Methionine Dependence of Cancer.Biomolecules · 2020Review
- Multi-omics characterization of the necrotrophic mycoparasite Saccharomycopsis schoenii.PLoS pathogens · 2019Article
- Involvement of Sulfur in the Biosynthesis of Essential Metabolites in Pathogenic Fungi of Animals, ParticularlyFrontiers in microbiology · 2019Review
- Regulatory Networks Governing Methionine Catabolism into Volatile Organic Sulfur-Containing Compounds in ClonostachysApplied and environmental microbiology · 2018Article
- Selenate sensitivity of a laeA mutant is restored by overexpression of the bZIP protein MetR in Aspergillus fumigatus.Fungal genetics and biology : FG & B · 2018Article
- MitoCPR-A surveillance pathway that protects mitochondria in response to protein import stress.Science (New York, N.Y.) · 2018Article
- Skp1 Independent Function of Cdc53/Cul1 in F-box Protein Homeostasis.PLoS genetics · 2015Article
16 more citing papers are in PubMed but not listed here.
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Saccharomyces cerevisiae SCF(Met30) ubiquitin-protein ligase controls cell cycle function and sulfur amino acid metabolism. We report here that the SCF(Met30 )complex mediates the transcriptional repression of the MET gene network by triggering degradation of the transcriptional activator Met4p when intracellular S-adenosylmethionine (AdoMet) increases. This AdoMet-induced Met4p degradation is dependent upon the 26S proteasome function. Unlike Met4p, the other components of the specific transcriptional activation complexes that are assembled upstream of the MET genes do not appear to be regulated at the protein level. We provide evidence that the interaction between Met4p and the F-box protein Met30p occurs irrespective of the level of intracellular AdoMet, suggesting that the timing of Met4p degradation is not controlled by its interaction with the SCF(Met30) complex. We also demonstrate that Met30p is a short-lived protein, which localizes within the nucleus. Furthermore, transcription of the MET30 gene is regulated by intracellular AdoMet levels and is dependent upon the Met4p transcription activation function. Thus Met4p appears to control its own degradation by regulating the amount of assembled SCF(Met30) ubiquitin ligase.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.