ArticleThe EMBO journal2000
Involvement of ezrin/moesin in de novo actin assembly on phagosomal membranes.
Article in The EMBO journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
65 citing papers in PubMed, 175 citations in OpenAlex.
- Syntaxin-2 balances phagocytic uptake and phagolysosomal clearance in macrophages.Journal of cell science · 2025Article
- Assays of Platelet SNARE-actin Interactions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Ezrin drives adaptation of monocytes to the inflamed lung microenvironment.Cell death & disease · 2024Article
- Molecular Relay Stations in Membrane Nanotubes: IRSp53 Involved in Actin-Based Force Generation.International journal of molecular sciences · 2023Article
- Review
- Novel CTRP8-RXFP1-JAK3-STAT3 axis promotes Cdc42-dependent actin remodeling for enhanced filopodia formation and motility in human glioblastoma cells.Molecular oncology · 2022Article
- Human Corneal Epithelial Cells Internalize Aspergillus flavus Spores by Actin-Mediated Endocytosis.Infection and immunity · 2021Article
- Better Together: Current Insights Into Phagosome-Lysosome Fusion.Frontiers in immunology · 2021Review
- Inflammatory Immune Cytokine TNF-α Modulates Ezrin Protein ActivationFrontiers in pharmacology · 2021Article
- Phagosomal F-Actin Retention by Cryptococcus gattii Induces Dendritic Cell Immunoparalysis.mBio · 2020Article
- Therapeutic Effects of Hyaluronic Acid in Peritonitis-Induced Sepsis in Mice.Shock (Augusta, Ga.) · 2020Article
- ERM Proteins at the Crossroad of Leukocyte Polarization, Migration and Intercellular Adhesion.International journal of molecular sciences · 2020Review
- Interaction betweenFrontiers in cellular and infection microbiology · 2017Article
- CLIC1 regulates dendritic cell antigen processing and presentation by modulating phagosome acidification and proteolysis.Biology open · 2016Article
- U-73122 reduces the cell growth in cultured MG-63 ostesarcoma cell line involving Phosphoinositide-specific Phospholipases C.SpringerPlus · 2016Article
- Ezrin-related Phosphoinositide pathway modifies RhoA and Rac1 in human osteosarcoma cell lines.Journal of cell communication and signaling · 2015Article
- Ezrin silencing remodulates the expression of Phosphoinositide-specific Phospholipase C enzymes in human osteosarcoma cell lines.Journal of cell communication and signaling · 2014Article
- Article
- Article
- Gonadotropin-releasing hormone receptor activates GTPase RhoA and inhibits cell invasion in the breast cancer cell line MDA-MB-231.BMC cancer · 2012Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The current study focuses on the molecular mechanisms responsible for actin assembly on a defined membrane surface: the phagosome. Mature phagosomes were surrounded by filamentous actin in vivo in two different cell types. Fluorescence microscopy was used to study in vitro actin nucleation/polymerization (assembly) on the surface of phagosomes isolated from J774 mouse macrophages. In order to prevent non-specific actin polymerization during the assay, fluorescent G-actin was mixed with thymosin beta4. The cytoplasmic side of phagosomes induced de novo assembly and barbed end growth of actin filaments. This activity varied cyclically with the maturation state of phagosomes, both in vivo and in vitro. Peripheral membrane proteins are crucial components of this actin assembly machinery, and we demonstrate a role for ezrin and/or moesin in this process. We propose that this actin assembly process facilitates phagosome/endosome aggregation prior to membrane fusion.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.