ArticleInvestigative ophthalmology & visual science2000
Galectin-3 is associated with the plasma membrane of lens fiber cells.
Article in Investigative ophthalmology & visual science, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 47 citations in OpenAlex.
- The Mammalian Ocular Lens in Focus: Development, Anatomy, Physiology, Transparency, Biomechanics, and Age-related Challenges.Journal of visualized experiments : JoVE · 2026Review
- Structure of the lens MP20 mediated adhesive junction.bioRxiv : the preprint server for biology · 2024Article
- Spatially Resolved Proteomic Analysis of the Lens Extracellular Diffusion Barrier.Investigative ophthalmology & visual science · 2021Article
- Interaction between Galectin-3 and Integrins Mediates Cell-Matrix Adhesion in Endothelial Cells and Mesenchymal Stem Cells.International journal of molecular sciences · 2021Article
- Galectins in the Pathogenesis of Cerebrovascular Accidents: An Overview.Journal of experimental neuroscience · 2019Review
- Role of Pore-Lining Residues in Defining the Rate of Water Conduction by Aquaporin-0.Biophysical journal · 2017Article
- Overview of electron crystallography of membrane proteins: crystallization and screening strategies using negative stain electron microscopy.Current protocols in protein science · 2013Article
- Adhesion/growth-regulatory galectins in the human eye: localization profiles and tissue reactivities as a standard to detect disease-associated alterations.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2012Article
- Microphakia and congenital cataract formation in a novel Lim2(C51R) mutant mouse.Molecular vision · 2011Article
- The prototypical H+/galactose symporter GalP assembles into functional trimers.Journal of molecular biology · 2010Article
- Apoptosis gene profiling reveals spatio-temporal regulated expression of the p53/Mdm2 pathway during lens development.Experimental eye research · 2009Article
- Noncanonical binding of calmodulin to aquaporin-0: implications for channel regulation.Structure (London, England : 1993) · 2008Article
- Transthyretin interacts with the lysosome-associated membrane protein (LAMP-1) in circulation.The Biochemical journal · 2004Article
- Developmental aspects of galectin-3 expression in the lens.Histochemistry and cell biology · 2003Article
- MP20, the second most abundant lens membrane protein and member of the tetraspanin superfamily, joins the list of ligands of galectin-3.BMC cell biology · 2001Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo discover proteins that have the potential to contribute to the tight packing of fiber cells in the lens.
methodsCrude fiber cell membranes were isolated from ovine lens cortex. Proteins were separated by two-dimensional gel electrophoresis, and selected protein spots identified by micro-sequencing. The identification of galectin-3 was confirmed by immunoblotting with a specific antibody. The association of galectin-3 with the fiber cell plasma membrane was investigated using immunofluorescence microscopy, solubilization trials with selected reagents, and immunoprecipitation to identify candidate ligands.
resultsA cluster of three protein spots with an apparent molecular weight of 31,000 and isoelectric points ranging between 7 and 8.5 were resolved and identified as galectin-3. This protein was associated peripherally with the fiber cell plasma membrane and interacted with MP20, an abundant intrinsic membrane protein that had been identified previously as a component of membrane junctions between fiber cells.
conclusionsThe detection of galectin-3 in the lens is a novel result and adds to the growing list of lens proteins with adhesive properties. Its location at the fiber cell membrane and its association with the junction-forming MP20 is consistent with a potential role in the development or maintenance of the tightly packed lens tissue architecture.
Indexed as
Identifiers
10634621PMC3917502W1481342808What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.