ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2000
Proteasome involvement and accumulation of ubiquitinated proteins in cerebellar granule neurons undergoing apoptosis.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 139 citations in OpenAlex.
- Trehalose Promotes Clearance of Proteotoxic Aggregation of Neurodegenerative Disease-Associated Aberrant Proteins.Molecular neurobiology · 2024Article
- Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity.The Journal of biological chemistry · 2018Article
- Removing endogenous tau does not prevent tau propagation yet reduces its neurotoxicity.The EMBO journal · 2015Article
- The role of the ubiquitin proteasome system in cerebellar development and medulloblastoma.Molecular brain · 2015Review
- Roscovitine is a proteostasis regulator that corrects the trafficking defect of F508del-CFTR by a CDK-independent mechanism.British journal of pharmacology · 2014Article
- Clostridium difficile toxin A attenuates Wnt/β-catenin signaling in intestinal epithelial cells.Infection and immunity · 2014Article
- TLR4 mediates the impairment of ubiquitin-proteasome and autophagy-lysosome pathways induced by ethanol treatment in brain.Cell death & disease · 2014Article
- Serine racemase: a key player in apoptosis and necrosis.Frontiers in synaptic neuroscience · 2014Review
- Trim17-mediated ubiquitination and degradation of Mcl-1 initiate apoptosis in neurons.Cell death and differentiation · 2013Article
- Manumycin A inhibits triple-negative breast cancer growth through LC3-mediated cytoplasmic vacuolation death.Cell death & disease · 2013Article
- Nerve growth factor and Alzheimer's disease: new facts for an old hypothesis.Molecular neurobiology · 2012Review
- Insulin-like growth factor-I mediates neuroprotection in proteasome inhibition-induced cytotoxicity in SH-SY5Y cells.Molecular and cellular neurosciences · 2011Article
- Development and validation of a screening assay for the evaluation of putative neuroprotective agents in the treatment of Parkinson's disease.Neurotoxicity research · 2011Article
- Trim17, a novel E3 ubiquitin-ligase, initiates neuronal apoptosis.Cell death and differentiation · 2010Article
- Lactacystin requires reactive oxygen species and Bax redistribution to induce mitochondria-mediated cell death.British journal of pharmacology · 2009Article
- Systemic administration of proteasome inhibitor protects against MPTP neurotoxicity in mice.Cellular and molecular neurobiology · 2009Article
- Cadmium-induced activation of stress signaling pathways, disruption of ubiquitin-dependent protein degradation and apoptosis in primary rat Sertoli cell-gonocyte cocultures.Toxicological sciences : an official journal of the Society of Toxicology · 2008Article
- Endoplasmic reticulum stress-induced cell death mediated by the proteasome.Cell death and differentiation · 2007Article
- Killer proteases and little strokes--how the things that do not kill you make you stronger.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2007Review
- The modular systems biology approach to investigate the control of apoptosis in Alzheimer's disease neurodegeneration.BMC neuroscience · 2006Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
We investigated the potential role of the ubiquitin proteolytic system in the death of cerebellar granule neurons induced by reduction of extracellular potassium. Inhibitors of proteasomal function block apoptosis if administered at onset of this process, but they do not exert such effect when added 2-3 hr later. The same inhibitors also prevent caspase-3 activity and calpain-caspase-3-mediated processing of tau protein, suggesting that proteasomes are involved upstream of the caspase activation. Although the proteasomes seem to play an early primary role in programmed cell death, we found that with progression of apoptosis, during the execution phase, a perturbation in normal ubiquitin-proteasome function occurs, and high levels of ubiquitinated proteins accumulate in the cytoplasm of dying cells. Such accumulation correlates with a progressive decline of proteasome chymotrypsin and trypsin-like activities and, to a lower extent, of postacidic-like activity. Both intracytoplasmic accumulation of ubiquitinated proteins and decline of proteasome function are reversed by the pan-caspase inhibitor Z-VAD-fmk. The decline in proteasome function is accompanied by, and likely attributable to, a marked and progressive decline of deubiquitinating activities. The finding that the proteasomes are early involved in apoptosis and that ubiquitinated proteins accumulate during this process prospect granule neurons as a model system aimed at correlating these events with neurodegenerative diseases.
Indexed as
Identifiers
10632588PMC6772397W1614363344What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.