Evidence map›Paper›PMID 10631986›Full record

ArticleProtein science : a publication of the Protein Society1999

Cloning and expression of kinesins from the thermophilic fungus Thermomyces lanuginosus.

R Sakowicz, S Farlow, L S Goldstein

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Protein science : a publication of the Protein Society, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
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  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

R SakowiczHoward Hughes Medical Institute, Department of Cellular and Molecular Medicine, School of Medicine, University of California, San Diego, La Jolla 92093-0683, USA.
S Farlow
L S Goldstein
University of California, San Diego · USHoward Hughes Medical Institute · US

Funding

GENETIC AND IMMUNOLOGIC ANALYSIS OF MICROTUBULE PROTEINSR01GM035252 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI GOLDSTEIN, LAWRENCE S · 1985 to 2006
$2.7M
NIGMS NIH HHS GM35252
6 · The paper itself

Abstract

The motor domain regions of three novel members of the kinesin superfamily TLKIF1, TLKIFC, and TLBIMC were identified in a thermophilic fungus Thermomyces lanuginosus. Based on sequence similarity, they were classified as members of the known kinesin families Unc104/KIF1, KAR3, and BIMC. TLKIF1 was subsequently expressed in Escherichia coli. The expression level was high, and the protein was mostly soluble, easy to purify, and enzymatically active. TLKIF1 is a monomeric kinesin motor, which in a gliding motility assay displays a robust plus-directed microtubule movement up to 2 microm/s. The discovery of TLKIF1 also demonstrates that a family of kinesin motors not previously found in fungi may in fact be used in this group of organisms.

Indexed as

Adenosine TriphosphatasesAmino Acid SequenceAscomycotaBase SequenceCloning, MolecularEscherichia coliFungal ProteinsGene ExpressionKinesinsMicrotubulesMolecular Sequence DataPhylogenySequence AlignmentAdenosine TriphosphatasesFungal ProteinsKinesinsTLBIMC protein, Thermomyces lanuginosusTLKIF1 protein, Thermomyces lanuginosusTLKIFC protein, Thermomyces lanuginosus

Identifiers

PMID10631986
PMCPMC2144245
OpenAlexW2069394995

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.