ArticlePlant physiology2000
Developmental regulation of monoterpene biosynthesis in the glandular trichomes of peppermint.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
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Who cites it
68 citing papers in PubMed, 241 citations in OpenAlex.
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- Integrated metabolomic and transcriptomic profiling uncovers a citrus terpene synthase withFrontiers in plant science · 2026Article
- Inhibitor treatment and subcellular localization analysis reveal the contribution of a cytosolic terpene synthase to the substantial release of anti-insect monoterpenes by Sphagneticola trilobata (L.) Pruski.Journal of advanced research · 2025Article
- Changes in Physiological Traits, Gene Expression and Phytochemical Profile of Mentha piperita in Response to Elicitor.Biochemical genetics · 2025Article
- De Novo Terpenes Emitted from Juvenile Leaves ofMolecules (Basel, Switzerland) · 2025Article
- AaMYC3 bridges the regulation of glandular trichome density and artemisinin biosynthesis in Artemisia annua.Plant biotechnology journal · 2025Article
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- Chromosome-level genome assembly provides insights into the genome evolution and functional importance of the phenylpropanoid-flavonoid pathway in Thymus mongolicus.BMC genomics · 2024Article
- Changes in Metabolite Profiling and Expression Levels of Key Genes Involved in the Terpenoid Biosynthesis Pathway in Garden Sage (Metabolites · 2023Article
- Inducing the Production of Secondary Metabolites by Foliar Application of Methyl Jasmonate in Peppermint.Plants (Basel, Switzerland) · 2023Article
- Transcriptomics Reveals the Molecular Basis for Methyl Jasmonate to Promote the Synthesis of Monoterpenoids inCurrent issues in molecular biology · 2023Article
- The non-specific lipid transfer protein McLTPII.9 ofFrontiers in plant science · 2023Article
- Chromosome-level genome assembly of Mentha longifolia L. reveals gene organization underlying disease resistance and essential oil traits.G3 (Bethesda, Md.) · 2022Article
- Essential Oil Content and Compositional Variability ofMolecules (Basel, Switzerland) · 2022Article
- Genetic Manipulation of Biosynthetic Pathways in Mint.Frontiers in plant science · 2022Review
- Functional Characterization and Structural Insights Into Stereoselectivity of Pulegone Reductase in Menthol Biosynthesis.Frontiers in plant science · 2021Article
- Comparative temporal metabolomics studies to investigate interspecies variation in three Ocimum species.Scientific reports · 2020Article
8 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monoterpene production in peppermint (Mentha x piperita L.) glandular trichomes is determined by the rate of biosynthesis, as determined by (14)CO(2) incorporation, and is restricted to leaves 12 to 20 d of age. Using oil glands isolated from peppermint leaves of different ages, in vitro assay of the eight sequential enzymes responsible for the biosynthesis of the principal monoterpene (-)-menthol indicated that all but one biosynthetic enzyme had a very similar developmental profile. Activities were highest in leaves 12 to 20 d of age, with a sharp peak centered at 15 d. The exception, (-)-menthone reductase, the last enzyme of the pathway, exhibited a later peak of activity, which was centered at approximately 21 d. The correlation between in vitro enzyme activity and the rate of biosynthesis measured in vivo suggests that monoterpene formation is controlled mainly by the coordinately regulated activity of the relevant biosynthetic enzymes. Developmental immunoblotting of limonene synthase, which catalyzes the committed step of the pathway, demonstrated a direct correlation between enzyme activity and enzyme protein, suggesting that the dynamic time course for the remaining pathway enzyme activities also reflects the corresponding protein levels. RNA-blot analyses indicated that the genes encoding enzymes of the early pathway steps are transcriptionally activated in a coordinated fashion, with a time course superimpossible with activity measurements and immunoblot data. These results demonstrating coincidental temporal changes in enzyme activities, enzyme protein level, and steady-state transcript abundances indicate that most of the monoterpene biosynthetic enzymes in peppermint are developmentally regulated at the level of gene expression.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.