ArticlePlant physiology2000
Antisense HEMA1 RNA expression inhibits heme and chlorophyll biosynthesis in arabidopsis.
Article in Plant physiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers.
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Who cites it
63 citing papers in PubMed.
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- Function of ALA Content in Porphyrin Metabolism Regulation ofInternational journal of molecular sciences · 2023Article
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- Transcriptome and Metabolome Analysis of a Late-Senescent Vegetable Soybean during Seed Development Provides New Insights into Degradation of Chlorophyll.Antioxidants (Basel, Switzerland) · 2022Article
- Dynamic transcriptome and network-based analysis of yellow leaf mutant Ginkgo biloba.BMC plant biology · 2022Article
- Physiological and transcriptome analysis reveals the differences in nitrate content between lamina and midrib of flue-cured tobacco.Scientific reports · 2022Article
- Transcription Profiles Reveal Age-Dependent Variations of Photosynthetic Properties and Sugar Metabolism in Grape Leaves (International journal of molecular sciences · 2022Article
- Cytological, Biochemical, and Transcriptomic Analyses of a Novel Yellow Leaf Variation in aGenes · 2021Article
- A genome wide transcriptional study ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Cold acclimation can specifically inhibit chlorophyll biosynthesis in young leaves of Pakchoi.BMC plant biology · 2021Article
- Perspectives on improving light distribution and light use efficiency in crop canopies.Plant physiology · 2021Article
- Effect of chlorophyll biosynthesis-related genes on the leaf color in Hosta (Hosta plantaginea Aschers) and tobacco (Nicotiana tabacum L.).BMC plant biology · 2021Article
3 more citing papers are in PubMed but not listed here.
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2 authors.
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Abstract
5-aminolevulinic acid (ALA) is a precursor in the biosynthesis of tetrapyrroles including chlorophylls and heme. The formation of ALA involves two enzymatic steps which take place in the chloroplast in plants. The first enzyme, glutamyl-tRNA reductase, and the second enzyme, glutamate-1-semialdehyde-2,1-aminomutase, are encoded by the nuclear HEMA and GSA genes, respectively. To assess the significance of the HEMA gene for chlorophyll and heme synthesis, transgenic Arabidopsis plants that expressed antisense HEMA1 mRNA from the constitutive cauliflower mosaic virus 35S promoter were generated. These plants exhibited varying degrees of chlorophyll deficiency, ranging from patchy yellow to total yellow. Analysis indicated that these plants had decreased levels of chlorophyll, non-covalently bound hemes, and ALA; their levels were proportional to the level of glutamyl-tRNA reductase expression and were inversely related to the levels of antisense HEMA transcripts. Plants that lacked chlorophyll failed to survive under normal growth conditions, indicating that HEMA gene expression is essential for growth.
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