ArticleThe Journal of cell biology2000
Hydrogen peroxide inhibition of nuclear protein import is mediated by the mitogen-activated protein kinase, ERK2.
Article in The Journal of cell biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 73 citations in OpenAlex.
- Thermal Stress and Nuclear Transport.Advances in experimental medicine and biology · 2024Review
- Cellular Stress Induces Nucleocytoplasmic Transport Deficits Independent of Stress Granules.Biomedicines · 2022Article
- Comparative Proteomics Analysis Reveals Unique Early Signaling Response ofInternational journal of molecular sciences · 2020Article
- HIKESHI silencing can enhance mild hyperthermia sensitivity in human oral squamous cell carcinoma HSC‑3 cells.International journal of molecular medicine · 2020Article
- Thioredoxin-related transmembrane protein 2 (TMX2) regulates the Ran protein gradient and importin-β-dependent nuclear cargo transport.Scientific reports · 2019Article
- Fluorescence-based quantification of nucleocytoplasmic transport.Methods (San Diego, Calif.) · 2019Article
- Conversion of graded phosphorylation into switch-like nuclear translocation via autoregulatory mechanisms in ERK signalling.Nature communications · 2016Article
- Posttranslational modification of Sirt6 activity by peroxynitrite.Free radical biology & medicine · 2015Article
- BGLF4 kinase modulates the structure and transport preference of the nuclear pore complex to facilitate nuclear import of Epstein-Barr virus lytic proteins.Journal of virology · 2015Article
- Disruption of the ran system by cysteine oxidation of the nucleotide exchange factor RCC1.Molecular and cellular biology · 2015Article
- Nuclear pore rearrangements and nuclear trafficking in cardiomyocytes from rat and human failing hearts.Cardiovascular research · 2015Article
- Cellular maintenance of nuclear protein homeostasis.Cellular and molecular life sciences : CMLS · 2014Review
- A pathway linking oxidative stress and the Ran GTPase system in progeria.Molecular biology of the cell · 2014Article
- Altered transcription factor trafficking in oxidatively-stressed neuronal cells.Biochimica et biophysica acta · 2012Article
- Nuclear-cytoplasmic trafficking of NTF2, the nuclear import receptor for the RanGTPase, is subjected to regulation.PloS one · 2012Article
- Nuclear transport: a switch for the oxidative stress-signaling circuit?Journal of signal transduction · 2012Article
- Mitogen activated protein kinase at the nuclear pore complex.Journal of cellular and molecular medicine · 2011Article
- Nuclear transport, oxidative stress, and neurodegeneration.International journal of clinical and experimental pathology · 2011Review
- Dissecting the signaling events that impact classical nuclear import and target nuclear transport factors.PloS one · 2009Article
- Phosphoproteomics reveals new ERK MAP kinase targets and links ERK to nucleoporin-mediated nuclear transport.Nature structural & molecular biology · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
H(2)O(2) alters gene expression in many cell types. Alterations in nuclear import of transcription factors or similar key proteins may be responsible for these changes. To investigate this possibility, a cytosolic nuclear import cocktail was treated with varying ¿H(2)O(2) and used in import assays. H(2)O(2) caused a dose- and time-dependent inhibition of import at concentrations as low as 100 microM. Catalase reversed this effect. H(2)O(2) treatment of permeablized cells did not affect import, suggesting that H(2)O(2) was acting on a cytosolic factor. Treatment of import cocktail with two different free radical generating systems had no effect, but treatment of permeablized cells inhibited import, suggesting H(2)O(2) works via a distinct process from hydroxyl or superoxide radicals. Pretreatment of import cocktail with genistein reversed the effect of H(2)O(2) on import. Western blotting revealed that H(2)O(2) activated ERK2. The specific MEK1/2 inhibitor, PD98059, completely blocked the effects of H(2)O(2) on import. Activated ERK2 mimicked H(2)O(2)'s effect on import. Immunocytochemistry revealed that H(2)O(2) treatment of whole cells increased cytosolic Ran/TC4 levels, an effect reversible by catalase or PD98059. These data demonstrate that H(2)O(2) inhibits nuclear protein import and that this effect is mediated by mitogen-activated protein (MAP) kinase activation, possibly by altering Ran/TC4 function.
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