ArticleMolecular and cellular biology2000
The regulator of the yeast proline utilization pathway is differentially phosphorylated in response to the quality of the nitrogen source.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 57 citations in OpenAlex.
- Functional Characterization ofMicroorganisms · 2022Article
- Longevity Regulation by Proline Oxidation in Yeast.Microorganisms · 2021Article
- Saccharomyces cerevisiae Gene Expression during Fermentation of Pinot Noir Wines at an Industrially Relevant Scale.Applied and environmental microbiology · 2021Article
- Yeast homologs of human MCUR1 regulate mitochondrial proline metabolism.Nature communications · 2020Article
- Parameter Estimation and Variable Selection for Big Systems of Linear Ordinary Differential Equations: A Matrix-Based Approach.Journal of the American Statistical Association · 2019Article
- Proline Accumulation Influenced by Osmotic Stress in Arbuscular Mycorrhizal Symbiotic Plants.Frontiers in microbiology · 2018Article
- Article
- Systematic Analysis of Transcriptional and Post-transcriptional Regulation of Metabolism in Yeast.PLoS computational biology · 2017Article
- Broad metabolic sensitivity profiling of a prototrophic yeast deletion collection.Genome biology · 2014Article
- L-Proline uptake in Saccharomyces cerevisiae mitochondria can contribute to bioenergetics during nutrient stress as alternative mitochondrial fuel.World journal of microbiology & biotechnology · 2014Article
- Article
- Expression variability of co-regulated genes differentiates Saccharomyces cerevisiae strains.BMC genomics · 2011Article
- Transcriptional activation of the general amino acid permease gene per1 by the histone deacetylase Clr6 Is regulated by Oca2 kinase.Molecular and cellular biology · 2010Article
- Mutation of a phosphorylatable residue in Put3p affects the magnitude of rapamycin-induced PUT1 activation in a Gat1p-dependent manner.The Journal of biological chemistry · 2009Article
- Article
- Global phenotype screening and transcript analysis outlines the inhibitory mode(s) of action of two amphibian-derived, alpha-helical, cationic peptides on Saccharomyces cerevisiae.Antimicrobial agents and chemotherapy · 2007Article
- Effect of 21 different nitrogen sources on global gene expression in the yeast Saccharomyces cerevisiae.Molecular and cellular biology · 2007Article
- A fungal family of transcriptional regulators: the zinc cluster proteins.Microbiology and molecular biology reviews : MMBR · 2006Review
- Synergistic operation of four cis-acting elements mediate high level DAL5 transcription in Saccharomyces cerevisiae.FEMS yeast research · 2004Article
- Modulation of transcription factor function by an amino acid: activation of Put3p by proline.The EMBO journal · 2003Article
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2 authors at 2 institutions in 1 country.
Funding
Abstract
The proline utilization pathway in Saccharomyces cerevisiae is regulated by the Put3p transcriptional activator in response to the presence of the inducer proline and the quality of the nitrogen source in the growth medium. Put3p is constitutively bound to the promoters of its target genes, PUT1 and PUT2, under all conditions studied but activates transcription to the maximum extent only in the absence of rich nitrogen sources and in the presence of proline (i.e., when proline serves as the sole source of nitrogen). Changes in target gene expression therefore occur through changes in the activity of the DNA-bound regulator. In this report, we demonstrate by phosphatase treatment of immunoprecipitates of extracts metabolically labeled with (32)P or (35)S that Put3p is a phosphoprotein. Examination of Put3p isolated from cells grown on a variety of nitrogen sources showed that it was differentially phosphorylated as a function of the quality of the nitrogen source: the poorer the nitrogen source, the slower the gel migration of the phosphoforms. The presence of the inducer does not detectably alter the phosphorylation profile. Activator-defective and activator-constitutive Put3p mutants have been analyzed. One activator-defective mutant appears to be phosphorylated in a pattern similar to that of the wild type, thus separating its ability to be phosphorylated from its ability to activate transcription. Three activator-constitutive mutant proteins from cells grown on an ammonia-containing medium have a phosphorylation profile similar to that of the wild-type protein in cells grown on proline. These results demonstrate a correlation between the phosphorylation status of Put3p and its ability to activate its target genes and suggest that there are two signals, proline induction and quality of nitrogen source, impinging on Put3p that act synergistically for maximum expression of the proline utilization pathway.
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