ArticleMolecular and cellular biology2000
Biochemical and genetic conservation of fission yeast Dsk1 and human SR protein-specific kinase 1.
Article in Molecular and cellular biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 32 citations in OpenAlex.
- Evolution of the Early Spliceosomal Complex-From Constitutive to Regulated Splicing.International journal of molecular sciences · 2021Review
- The Dual-Specificity LAMMER Kinase Affects Stress-Response and Morphological Plasticity in Fungi.Frontiers in cellular and infection microbiology · 2019Review
- Genetic interaction mapping reveals a role for the SWI/SNF nucleosome remodeler in spliceosome activation in fission yeast.PLoS genetics · 2015Article
- Molecular characterization and expression patterns of serine/arginine-rich specific kinase 3 (SPRK3) in porcine skeletal muscle.Molecular biology reports · 2011Article
- Bruchpilot in ribbon-like axonal agglomerates, behavioral defects, and early death in SRPK79D kinase mutants of Drosophila.PLoS genetics · 2009Article
- Dsk1p kinase phosphorylates SR proteins and regulates their cellular localization in fission yeast.The Biochemical journal · 2007Article
- Regulated cellular partitioning of SR protein-specific kinases in mammalian cells.Molecular biology of the cell · 2006Article
- trans and cis splicing in trypanosomatids: mechanism, factors, and regulation.Eukaryotic cell · 2003Review
- Pre-mRNA splicing in Schizosaccharomyces pombe: regulatory role of a kinase conserved from fission yeast to mammals.Current genetics · 2003Review
- Interactions between two fission yeast serine/arginine-rich proteins and their modulation by phosphorylation.The Biochemical journal · 2002Article
- Human autoimmune sera as molecular probes for the identification of an autoantigen kinase signaling pathway.The Journal of experimental medicine · 2002Article
- Evidence for a role of Sky1p-mediated phosphorylation in 3' splice site recognition involving both Prp8 and Prp17/Slu4.RNA (New York, N.Y.) · 2001Article
- Fission yeast Prp4p kinase regulates pre-mRNA splicing by phosphorylating a non-SR-splicing factor.EMBO reports · 2001Article
- Analysis of the splicing machinery in fission yeast: a comparison with budding yeast and mammals.Nucleic acids research · 2000Review
- Gene ontology: tool for the unification of biology. The Gene Ontology Consortium.Nature genetics · 2000Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Arginine/serine-rich (RS) domain-containing proteins and their phosphorylation by specific protein kinases constitute control circuits to regulate pre-mRNA splicing and coordinate splicing with transcription in mammalian cells. We present here the finding that similar SR networks exist in Schizosaccharomyces pombe. We previously showed that Dsk1 protein, originally described as a mitotic regulator, displays high activity in phosphorylating S. pombe Prp2 protein (spU2AF59), a homologue of human U2AF65. We now demonstrate that Dsk1 also phosphorylates two recently identified fission yeast proteins with RS repeats, Srp1 and Srp2, in vitro. The phosphorylated proteins bear the same phosphoepitope found in mammalian SR proteins. Consistent with its substrate specificity, Dsk1 forms kinase-competent complexes with those proteins. Furthermore, dsk1(+) gene determines the phenotype of prp2(+) overexpression, providing in vivo evidence that Prp2 is a target for Dsk1. The dsk1-null mutant strain became severely sick with the additional deletion of a related kinase gene. Significantly, human SR protein-specific kinase 1 (SRPK1) complements the growth defect of the double-deletion mutant. In conjunction with the resemblance of dsk1(+) and SRPK1 in sequence homology, biochemical properties, and overexpression phenotypes, the complementation result indicates that SRPK1 is a functional homologue of Dsk1. Collectively, our studies illustrate the conserved SR networks in S. pombe consisting of RS domain-containing proteins and SR protein-specific kinases and thus establish the importance of the networks in eucaryotic organisms.
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