ArticleGenetics2000
Quantitative analysis of gene function in the Drosophila embryo.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
51 citing papers in PubMed, 101 citations in OpenAlex.
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- Recurrent Duplication and Diversification of a Vital DNA Repair Gene Family Across Drosophila.Molecular biology and evolution · 2024Article
- Metabolic regulation of proteome stability via N-terminal acetylation controls male germline stem cell differentiation and reproduction.Nature communications · 2023Article
- Article
- A clinically-relevant residue of POLR1D is required for Drosophila development.Developmental dynamics : an official publication of the American Association of Anatomists · 2022Article
- A conditional GABAergic synaptic vesicle marker for Drosophila.Journal of neuroscience methods · 2022Article
- A conditional glutamatergic synaptic vesicle marker for Drosophila.G3 (Bethesda, Md.) · 2022Article
- Distinct gene expression dynamics in germ line and somatic tissue during ovariole morphogenesis in Drosophila melanogaster.G3 (Bethesda, Md.) · 2022Article
- The Tudor Domain-Containing Protein, Kotsubu (CG9925), Localizes to the Nuage and Functions in piRNA Biogenesis inFrontiers in molecular biosciences · 2022Article
- A dual role for DNA binding by Runt in activation and repression ofMolecular biology of the cell · 2021Article
- Maternally inherited intron coordinates primordial germ cell homeostasis during Drosophila embryogenesis.Cell death and differentiation · 2021Article
- Mutations in the insulator protein Suppressor of Hairy wing induce genome instability.Chromosoma · 2020Article
- Demonstration of a Simple Epitope Tag Multimerization Strategy for Enhancing the Sensitivity of Protein Detection UsingG3 (Bethesda, Md.) · 2020Article
- Two-By-One model of cytoplasmic incompatibility: Synthetic recapitulation by transgenic expression of cifA and cifB in Drosophila.PLoS genetics · 2019Article
- RUNX3 regulates cell cycle-dependent chromatin dynamics by functioning as a pioneer factor of the restriction-point.Nature communications · 2019Article
- Conditional Synaptic Vesicle Markers forG3 (Bethesda, Md.) · 2019Article
- Dynamic patterning by the Drosophila pair-rule network reconciles long-germ and short-germ segmentation.PLoS biology · 2017Article
- nanos-Driven expression of piggyBac transposase induces mobilization of a synthetic autonomous transposon in the malaria vector mosquito, Anopheles stephensi.Insect biochemistry and molecular biology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The specific functions of gene products frequently depend on the developmental context in which they are expressed. Thus, studies on gene function will benefit from systems that allow for manipulation of gene expression within model systems where the developmental context is well defined. Here we describe a system that allows for genetically controlled overexpression of any gene of interest under normal physiological conditions in the early Drosophila embryo. This regulated expression is achieved through the use of Drosophila lines that express a maternal mRNA for the yeast transcription factor GAL4. Embryos derived from females that express GAL4 maternally activate GAL4-dependent UAS transgenes at uniform levels throughout the embryo during the blastoderm stage of embryogenesis. The expression levels can be quantitatively manipulated through the use of lines that have different levels of maternal GAL4 activity. Specific phenotypes are produced by expression of a number of different developmental regulators with this system, including genes that normally do not function during Drosophila embryogenesis. Analysis of the response to overexpression of runt provides evidence that this pair-rule segmentation gene has a direct role in repressing transcription of the segment-polarity gene engrailed. The maternal GAL4 system will have applications both for the measurement of gene activity in reverse genetic experiments as well as for the identification of genetic factors that have quantitative effects on gene function in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.