ArticleGenetics2000
Isolation and characterization of Nrf1p, a novel negative regulator of the Cdc42p GTPase in Schizosaccharomyces pombe.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- Optogenetics reveals Cdc42 local activation by scaffold-mediated positive feedback and Ras GTPase.PLoS biology · 2020Article
- Reconstruction of signaling networks regulating fungal morphogenesis by transcriptomics.Eukaryotic cell · 2009Article
- Ablation of a small transmembrane protein of Trypanosoma brucei (TbVTC1) involved in the synthesis of polyphosphate alters acidocalcisome biogenesis and function, and leads to a cytokinesis defect.The Biochemical journal · 2007Article
- The vacuolar transporter chaperone (VTC) complex is required for microautophagy.Molecular biology of the cell · 2007Article
- Spatial segregation of Ras signaling: new evidence from fission yeast.Cell cycle (Georgetown, Tex.) · 2006Review
- Compartmentalized signaling of Ras in fission yeast.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Schizosaccharomyces pombe Ras1 effector, Scd1, interacts with Klp5 and Klp6 kinesins to mediate cytokinesis.Genetics · 2003Article
- Remodeling of organelle-bound actin is required for yeast vacuole fusion.The Journal of cell biology · 2002Article
- Saccharomyces cerevisiae Cdc42p localizes to cellular membranes and clusters at sites of polarized growth.Eukaryotic cell · 2002Article
- Yeast vacuoles and membrane fusion pathways.The EMBO journal · 2002Review
- The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation.The EMBO journal · 2002Article
- Rho1p and Cdc42p act after Ypt7p to regulate vacuole docking.The EMBO journal · 2001Article
- Cdc42p functions at the docking stage of yeast vacuole membrane fusion.The EMBO journal · 2001Article
- New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis.Molecular biology of the cell · 2000Article
- Fission yeast Ras1 effector Scd1 interacts with the spindle and affects its proper formation.Genetics · 2000Article
- A complex of N-WASP and WIP integrates signalling cascades that lead to actin polymerization.Nature cell biology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Cdc42p GTPase and its regulators, such as the Saccharomyces cerevisiae Cdc24p guanine-nucleotide exchange factor, control signal-transduction pathways in eukaryotic cells leading to actin rearrangements. A cross-species genetic screen was initiated based on the ability of negative regulators of Cdc42p to reverse the Schizosaccharomyces pombe Cdc42p suppression of a S. cerevisiae cdc24(ts) mutant. A total of 32 S. pombe nrf (negative regulator of Cdc forty two) cDNAs were isolated that reversed the suppression. One cDNA, nrf1(+), encoded an approximately 15 kD protein with three potential transmembrane domains and 78% amino-acid identity to a S. cerevisiae gene, designated NRF1. A S. pombe Deltanrf1 mutant was viable but overexpression of nrf1(+) in S. pombe resulted in dose-dependent lethality, with cells exhibiting an ellipsoidal morphology indicative of loss of polarized cell growth along with partially delocalized cortical actin and large vacuoles. nrf1(+) also displayed synthetic overdose phenotypes with cdc42 and pak1 alleles. Green fluorescent protein (GFP)-Cdc42p and GFP-Nrf1p colocalized to intracellular membranes, including vacuolar membranes, and to sites of septum formation during cytokinesis. GFP-Nrf1p vacuolar localization depended on the S. pombe Cdc24p homolog Scd1p. Taken together, these data are consistent with Nrf1p functioning as a negative regulator of Cdc42p within the cell polarity pathway.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.