ArticleGenetics2000
Extensive genetic interactions between PRP8 and PRP17/CDC40, two yeast genes involved in pre-mRNA splicing and cell cycle progression.
Article in Genetics, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
- Whole-exome profiles of inflammatory breast cancer and pathological response to neoadjuvant chemotherapy.Journal of translational medicine · 2024Article
- An Arabidopsis pre-RNA processing8a (prp8a) missense allele restores splicing of a subset of mis-spliced mRNAs.Plant physiology · 2022Article
- Therapy-induced stress response is associated with downregulation of pre-mRNA splicing in cancer cells.Genome medicine · 2018Article
- HBx-induced MiR-1269b in NF-κB dependent manner upregulates cell division cycle 40 homolog (CDC40) to promote proliferation and migration in hepatoma cells.Journal of translational medicine · 2016Article
- Repurposed Transcriptomic Data Reveal Small Viral RNA Produced by Influenza Virus during Infection in Mice.PloS one · 2016Article
- Article
- Graded requirement for the spliceosome in cell cycle progression.Cell cycle (Georgetown, Tex.) · 2015Article
- Transcript maturation in apicomplexan parasites.Current opinion in microbiology · 2014Review
- CEF1/CDC5 alleles modulate transitions between catalytic conformations of the spliceosome.RNA (New York, N.Y.) · 2012Article
- PRPF mutations are associated with generalized defects in spliceosome formation and pre-mRNA splicing in patients with retinitis pigmentosa.Human molecular genetics · 2011Article
- Suppressors of the cdc-25.1(gf)-associated intestinal hyperplasia reveal important maternal roles for prp-8 and a subset of splicing factors in C. elegans.RNA (New York, N.Y.) · 2008Article
- A role for the yeast cell cycle/splicing factor Cdc40 in the G1/S transition.Current genetics · 2007Article
- Exon ligation is proofread by the DExD/H-box ATPase Prp22p.Nature structural & molecular biology · 2006Article
- Specific role of the SR protein splicing factor B52 in cell cycle control in Drosophila.Molecular and cellular biology · 2006Article
- Ubiquitin binding by a variant Jab1/MPN domain in the essential pre-mRNA splicing factor Prp8p.RNA (New York, N.Y.) · 2006Article
- Prp8 protein: at the heart of the spliceosome.RNA (New York, N.Y.) · 2005Review
- The Saccharomyces cerevisiae gene CDC40/PRP17 controls cell cycle progression through splicing of the ANC1 gene.Nucleic acids research · 2004Article
- Mutations in U5 snRNA loop 1 influence the splicing of different genes in vivo.Nucleic acids research · 2002Article
- Mutations in genes of Saccharomyces cerevisiae encoding pre-mRNA splicing factors cause cell cycle arrest through activation of the spindle checkpoint.Nucleic acids research · 2002Article
- Characterization of interactions among the Cef1p-Prp19p-associated splicing complex.RNA (New York, N.Y.) · 2002Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biochemical and genetic experiments have shown that the PRP17 gene of the yeast Saccharomyces cerevisiae encodes a protein that plays a role during the second catalytic step of the splicing reaction. It was found recently that PRP17 is identical to the cell division cycle CDC40 gene. cdc40 mutants arrest at the restrictive temperature after the completion of DNA replication. Although the PRP17/CDC40 gene product is essential only at elevated temperatures, splicing intermediates accumulate in prp17 mutants even at the permissive temperature. In this report we describe extensive genetic interactions between PRP17/CDC40 and the PRP8 gene. PRP8 encodes a highly conserved U5 snRNP protein required for spliceosome assembly and for both catalytic steps of the splicing reaction. We show that mutations in the PRP8 gene are able to suppress the temperature-sensitive growth phenotype and the splicing defect conferred by the absence of the Prp17 protein. In addition, these mutations are capable of suppressing certain alterations in the conserved PyAG trinucleotide at the 3' splice junction, as detected by an ACT1-CUP1 splicing reporter system. Moreover, other PRP8 alleles exhibit synthetic lethality with the absence of Prp17p and show a reduced ability to splice an intron bearing an altered 3' splice junction. On the basis of these findings, we propose a model for the mode of interaction between the Prp8 and Prp17 proteins during the second catalytic step of the splicing reaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.