ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2000
Antibodies to the estrogen receptor-alpha modulate rapid prolactin release from rat pituitary tumor cells through plasma membrane estrogen receptors.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 129 citations in OpenAlex.
- The Emerging Role of Prolactin as a Biomarker for Reproduction, Health, and Welfare in Wildlife Species: Elephants as a Model.Advances in experimental medicine and biology · 2026Review
- Effects of isoflavone supplementation, via red clover hay, on the growth and postgraze physiological recovery of beef steers grazing endophyte-infected tall fescue pastures.Journal of animal science · 2025Article
- Exploration of the Effects of TGF-Contrast media & molecular imaging · 2022Article
- Immunomodulation of carcinogens-induced steroids-dependent human diseases.Saudi journal of biological sciences · 2019Review
- Extranuclear signaling by sex steroid receptors and clinical implications in breast cancer.Molecular and cellular endocrinology · 2018Review
- The Effects of Steroid Implant and Dietary Soybean Hulls on Estrogenic Activity of Sera of Steers Grazing Toxic Endophyte-Infected Tall Fescue Pasture.Frontiers in veterinary science · 2015Article
- Article
- The role of estrogen, progesterone and aromatase in human non-small-cell lung cancer.Lung cancer management · 2012Article
- Non-genomic effects of xenoestrogen mixtures.International journal of environmental research and public health · 2012Review
- Estrogens induce expression of membrane-associated estrogen receptor α isoforms in lactotropes.PloS one · 2012Article
- Endocrine disruption via estrogen receptors that participate in nongenomic signaling pathways.The Journal of steroid biochemistry and molecular biology · 2011Review
- Progesterone and estrogen receptor expression and activity in human non-small cell lung cancer.Steroids · 2011Article
- Combinations of physiologic estrogens with xenoestrogens alter ERK phosphorylation profiles in rat pituitary cells.Environmental health perspectives · 2011Article
- Combinations of physiologic estrogens with xenoestrogens alter calcium and kinase responses, prolactin release, and membrane estrogen receptor trafficking in rat pituitary cells.Environmental health : a global access science source · 2010Article
- Estrogens of multiple classes and their role in mental health disease mechanisms.International journal of women's health · 2010Article
- Article
- 17beta-Estradiol-induced enhancement of estrogen receptor biosynthesis via MAPK pathway in mouse skeletal muscle myoblasts.The journal of physiological sciences : JPS · 2009Article
- Nongenomic actions of estradiol compared with estrone and estriol in pituitary tumor cell signaling and proliferation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2008Article
- Estrogen induces rapid translocation of estrogen receptor beta, but not estrogen receptor alpha, to the neuronal plasma membrane.Neuroscience · 2008Article
- Nongenomic actions of low concentration estrogens and xenoestrogens on multiple tissues.Molecular and cellular endocrinology · 2007Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
Antibodies (Abs) raised against the estrogen receptor-alpha (ERalpha) were used to investigate the role of ERalpha proteins located at the plasma membrane in mediating the rapid, estrogen-stimulated secretion of prolactin (PRL) from rat pituitary GH(3)/B6/F10 cells. Exposure of the cells to 1 nM 17beta-estradiol (E(2)) significantly increased PRL release after 3 or 6 min. When ERalpha Abs that bind specifically to ERalpha but are too large to diffuse into cells were tested for activity at the cell membrane, Ab R4, targeted to an ERalpha hinge region sequence, increased PRL release in a time- and concentration-dependent fashion. Ab H151, directed against a different hinge region epitope, decreased PRL release and blocked the stimulatory action of E(2). Abs raised against the DNA binding domain (H226) or the carboxyl terminus (C542) were not biologically active. When each Ab was examined for recognition of ERalpha on the cell surface by immunocytochemistry, all except H151 generated immunostaining in aldehyde-fixed cells. In live cells, however, Ab H151 but not Ab R4 blocked the membrane binding of fluorescently tagged E(2)-BSA. Overall, the data indicate that plasma membrane ERalpha proteins mediate estrogen-stimulated PRL release from GH(3)/B6/F10 cells. These results may also convey information about conformationally sensitive areas of the membrane form of ERalpha involved in rapid, nongenomic responses to estrogens.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.