ArticleJournal of virology2000
Expression of the Totivirus Helminthosporium victoriae 190S virus RNA-dependent RNA polymerase from its downstream open reading frame in dicistronic constructs.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 39 citations in OpenAlex.
- Genomic Characterization of a Novel Victorivirus Detected in a Sample Derived From a Human-Biting Tick in Japan.Microbiology and immunology · 2026Article
- Infection pattern of male-killing viruses alters phenotypes in the tea tortrix moth Homona magnanima.Heredity · 2025Article
- Molecular characterization of birch toti-like virus, a plant-associated member of the new family Orthototiviridae.Archives of virology · 2024Article
- Identification and genetic characterization of a new totivirus from Bursera graveolens in western Ecuador.Archives of virology · 2023Article
- Capsid Structure of dsRNA Fungal Viruses.Viruses · 2018Review
- Three-dimensional structure of victorivirus HvV190S suggests coat proteins in most totiviruses share a conserved core.PLoS pathogens · 2013Article
- RNA sequence determinants of a coupled termination-reinitiation strategy for downstream open reading frame translation in Helminthosporium victoriae virus 190S and other victoriviruses (Family Totiviridae).Journal of virology · 2011Article
- Coupled termination/reinitiation for translation of the downstream open reading frame B of the prototypic hypovirus CHV1-EP713.Nucleic acids research · 2009Article
- Cloning and characterization of a totivirus double-stranded RNA from the plant pathogenic fungus, Helicobasidium mompa Tanaka.Virus genes · 2003Article
- Essential and dispensable virus-encoded replication elements revealed by efforts To develop hypoviruses as gene expression vectors.Journal of virology · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The undivided double-stranded RNA (dsRNA) genome of Helminthosporium victoriae 190S virus (Hv190SV) (genus Totivirus) consists of two large overlapping open reading frames (ORFs). The 5'-proximal ORF encodes a capsid protein (CP), and the downstream, 3'-proximal ORF encodes an RNA-dependent RNA polymerase (RDRP). Unlike the RDRPs of some other totiviruses, which are expressed as a CP-RDRP (Gag-Pol-like) fusion protein, the Hv190SV RDRP is detected only as a separate, nonfused polypeptide. In this study, we examined the expression of the RDRP ORF fused in frame to the coding sequence of the green fluorescent protein (GFP) in bacteria and Schizosaccharomyces pombe cells. The GFP fusions were readily detected in bacteria transformed with the monocistronic construct RDRP:GFP; expression of the downstream RDRP:GFP from the dicistronic construct CP-RDRP:GFP could not be detected. However, fluorescence microscopy and Western blot analysis indicated that RDRP:GFP was expressed at low levels from its downstream ORF in the dicistronic construct in S. pombe cells. No evidence that the RDRP ORF was expressed from a transcript shorter than the full-length dicistronic mRNA was found. A coupled termination-reinitiation mechanism that requires host or eukaryotic cell factors is proposed for the expression of Hv190SV RDRP.
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