ArticleJournal of virology2000
The nuclear export signal within the E4orf6 protein of adenovirus type 5 supports virus replication and cytoplasmic accumulation of viral mRNA.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 77 citations in OpenAlex.
- Enhanced AAV production via rational design of a novel pHelper vector integrated with HSV-1 helper genes.Synthetic and systems biotechnology · 2026Article
- Breaking barriers: Respiratory viral strategies targeting the host's nuclear pore complex and nuclear transport pathways.Molecular biology of the cell · 2026Review
- Development of a novel adenovirus type 4 vector as a promising respiratory vaccine vehicle.Frontiers in immunology · 2025Article
- The E3 ubiquitin ligase activity associated with the adenoviral E1B-55K-E4orf6 complex does not require CRM1-dependent export.Journal of virology · 2011Article
- HuR keeps an angiogenic switch on by stabilising mRNA of VEGF and COX-2 in tumour endothelium.British journal of cancer · 2011Article
- Infection with E1B-mutant adenovirus stabilizes p53 but blocks p53 acetylation and activity through E1A.Oncogene · 2011Article
- Adenoviral producer cells.Viruses · 2010Article
- HuR is exported to the cytoplasm in oral cancer cells in a different manner from that of normal cells.British journal of cancer · 2009Article
- Adenovirus ubiquitin-protein ligase stimulates viral late mRNA nuclear export.Journal of virology · 2007Article
- Enhanced cytotoxicity without internuclear spread of adenovirus upon cell fusion by measles virus glycoproteins.Journal of virology · 2005Article
- Both BC-box motifs of adenovirus protein E4orf6 are required to efficiently assemble an E3 ligase complex that degrades p53.Molecular and cellular biology · 2004Article
- Nuclear export of the nonenveloped parvovirus virion is directed by an unordered protein signal exposed on the capsid surface.Journal of virology · 2004Article
- E1B-55-kilodalton protein is not required to block p53-induced transcription during adenovirus infection.Journal of virology · 2004Article
- Viral regulation of mRNA export.Journal of virology · 2004Article
- Article
- Adenovirus E1B 55-kilodalton oncoprotein binds to Daxx and eliminates enhancement of p53-dependent transcription by Daxx.Journal of virology · 2003Article
- Nuclear export of adenovirus E4orf6 protein is necessary for its ability to antagonize apoptotic activity of BH3-only proteins.Oncogene · 2003Article
- Differential regulation of p63 and p73 expression.Oncogene · 2003Article
- Mutual interference of adenovirus infection and myc expression.Journal of virology · 2003Article
- An activity associated with human chromosome 21 permits nuclear colocalization of the adenovirus E1B-55K and E4orf6 proteins and promotes viral late gene expression.Journal of virology · 2003Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During the late phase of adenovirus infection, viral mRNA is efficiently transported from the nucleus to the cytoplasm while most cellular mRNA species are retained in the nucleus. Two viral proteins, E1B-55 kDa and E4orf6, are both necessary for these effects. The E4orf6 protein of adenovirus type 5 binds and relocalizes E1B-55 kDa, and the complex of the two proteins was previously shown to shuttle continuously between the nucleus and cytoplasm. Nucleocytoplasmic transport of the complex is achieved by a nuclear export signal (NES) within E4orf6. Mutation of this signal sequence severely reduces the ability of the E1B-55 kDa-E4orf6 complex to leave the nucleus. Here, we examined the role of functional domains within E4orf6 during virus infection. E4orf6 or mutants derived from it were transiently expressed, followed by infection with recombinant adenovirus lacking the E4 region and determination of virus yield. An arginine-rich putative alpha helix near the carboxy terminus of E4orf6 contributes to E1B-55 kDa binding and relocalization as well as to the synthesis of viral DNA, mRNA, and proteins. Further mutational analysis revealed that mutation of the NES within E4orf6 considerably reduces its ability to support virus production. The same effect was observed when nuclear export was blocked with a competitor. Further, a functional NES within E4orf6 contributed to the efficiency of late virus protein synthesis and viral DNA replication, as well as total and cytoplasmic accumulation of viral late mRNA. Our data support the view that NES-mediated nucleocytoplasmic shuttling strongly enhances most, if not all, intracellular activities of E4orf6 during the late phase of adenovirus infection.
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