ArticleJournal of virology2000
Mutations of the kissing-loop dimerization sequence influence the site specificity of murine leukemia virus recombination in vivo.
Article in Journal of virology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Beyond reverse transcription: molecular mechanisms and emerging paradigms in retroviral replication.FEMS microbiology reviews · 2026Review
- Two Peas in a Pod: Retroviral RNA Dimers Organize Gag-RNA Nanoclusters with Novel Biophysical Properties.International journal of molecular sciences · 2025Article
- Two peas in a pod: retroviral RNA dimers organize Gag-RNA nanoclusters with novel biophysical properties.bioRxiv : the preprint server for biology · 2025Article
- Effect of the SARS-CoV-2 Delta-associated G15U mutation on the s2m element dimerization and its interactions with miR-1307-3p.RNA (New York, N.Y.) · 2023Article
- Retroviral RNA Dimerization: From Structure to Functions.Frontiers in microbiology · 2018Review
- Recombination of 5' subgenomic RNA3a with genomic RNA3 of Brome mosaic bromovirus in vitro and in vivo.Virology · 2011Article
- Murine leukemia virus RNA dimerization is coupled to transcription and splicing processes.Retrovirology · 2010Article
- Characterization of a natural heterodimer between MLV genomic RNA and the SD' retroelement generated by alternative splicing.RNA (New York, N.Y.) · 2007Article
- HIV-2 RNA dimerization is regulated by intramolecular interactions in vitro.RNA (New York, N.Y.) · 2007Article
- Local mutagenic impact of insertions of LTR retrotransposons on the mouse genome.Journal of molecular evolution · 2006Article
- The key role for local base order in the generation of multiple forms of China HIV-1 B'/C intersubtype recombinants.BMC evolutionary biology · 2005Article
- An RNA secondary structure bias for non-homologous reverse transcriptase-mediated deletions in vivo.Nucleic acids research · 2004Article
- Complementarity-directed RNA dimer-linkage promotes retroviral recombination in vivo.Nucleic acids research · 2004Article
- Template dimerization promotes an acceptor invasion-induced transfer mechanism during human immunodeficiency virus type 1 minus-strand synthesis.Journal of virology · 2003Article
- Dimerization and template switching in the 5' untranslated region between various subtypes of human immunodeficiency virus type 1.Journal of virology · 2003Article
- In vitro intersubtype recombinants of human immunodeficiency virus type 1: comparison to recent and circulating in vivo recombinant forms.Journal of virology · 2002Article
- Effects of varying sequence similarity on the frequency of repeat deletion during reverse transcription of a human immunodeficiency virus type 1 vector.Journal of virology · 2002Article
- Functional analysis of the murine sarcoma virus RNA packaging sequence.Journal of virology · 2002Article
- Duplication of the primary encapsidation and dimer linkage region of human immunodeficiency virus type 1 RNA results in the appearance of monomeric RNA in virions.Journal of virology · 2001Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The genetic information of retroviruses is retained within a dimeric RNA genome held together by intermolecular RNA-RNA interactions near the 5' ends. Coencapsidation of retrovirus-derived RNA molecules allows frequent template switching of the virus-encoded reverse transcriptase during DNA synthesis in newly infected cells. We have previously shown that template shifts within the 5' leader of murine leukemia viruses occur preferentially within the kissing stem-loop motif, a cis element crucial for in vitro RNA dimer formation. By use of a forced recombination approach based on single-cycle transfer of Akv murine leukemia virus-based vectors harboring defective primer binding site sequences, we now report that modifications of the kissing-loop structure, ranging from a deletion of the entire sequence to introduction of a single point mutation in the loop motif, significantly disturb site specificity of recombination within the highly structured 5' leader region. In addition, we find that an intact kissing-loop sequence favors optimal RNA encapsidation and vector transduction. Our data are consistent with the kissing-loop dimerization model and suggest that a direct intermolecular RNA-RNA interaction, here mediated by palindromic loop sequences within the mature genomic RNA dimer, facilitates hotspot template switching during retroviral cDNA synthesis in vivo.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.