ArticleTransgenic research1999
Apoplastic and cytosolic expression of full-size antibodies and antibody fragments in Nicotiana tabacum.
Article in Transgenic research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 121 citations in OpenAlex.
- Streamlined molecular farming of plant virus therapeutics for space flight and other low-resource environments.NPJ science of plants · 2026Article
- The landscape of antibody production systems: recombinant expression for research, diagnostics and therapy.Frontiers in bioengineering and biotechnology · 2026Review
- High-yield, plant-based production of an antimicrobial peptide with potent activity in a mouse model.Plant biotechnology journal · 2024Article
- Plant production of high affinity nanobodies that block SARS-CoV-2 spike protein binding with its receptor, human angiotensin converting enzyme.Frontiers in bioengineering and biotechnology · 2022Article
- Expression of Single Chain Variable Fragment (scFv) Molecules in Plants: A Comprehensive Update.Molecular biotechnology · 2020Review
- Transient Expression of Biologically Active Anti-rabies Virus Monoclonal Antibody in Tobacco Leaves.Iranian journal of biotechnology · 2018Article
- Nanobody-mediated resistance to Grapevine fanleaf virus in plants.Plant biotechnology journal · 2018Article
- Novel Bioengineered Cassava Expressing an Archaeal Starch Degradation System and a Bacterial ADP-Glucose Pyrophosphorylase for Starch Self-Digestibility and Yield Increase.Frontiers in plant science · 2018Article
- Reducing biomass recalcitrance by heterologous expression of a bacterial peroxidase in tobacco (Nicotiana benthamiana).Scientific reports · 2017Article
- A plant protein signal sequence improved humoral immune response to HPV prophylactic and therapeutic DNA vaccines.Human vaccines & immunotherapeutics · 2017Article
- Plant-derived SAC domain of PAR-4 (Prostate Apoptosis Response 4) exhibits growth inhibitory effects in prostate cancer cells.Frontiers in plant science · 2015Article
- Expression of recombinant antibody (single chain antibody fragment) in transgenic plant Nicotiana tabacum cv. Xanthi.Molecular biology reports · 2013Article
- Expression of the truncated tissue plasminogen activator (K2S) gene in tobacco chloroplast.Molecular biology reports · 2013Article
- Article
- Functional characterization of the recombinant HIV-neutralizing monoclonal antibody 2F5 produced in maize seeds.Plant molecular biology · 2012Article
- Expression and subcellular targeting of human complement factor C5a in Nicotiana species.PloS one · 2012Article
- High-level transient expression of ER-targeted human interleukin 6 in Nicotiana benthamiana.PloS one · 2012Article
- Glucose lowering effect of transgenic human insulin-like growth factor-I from rice: in vitro and in vivo studies.BMC biotechnology · 2011Article
- High-level expression of Camelid nanobodies in Nicotiana benthamiana.Transgenic research · 2010Article
- Expression and subcellular targeting of human insulin-like growth factor binding protein-3 in transgenic tobacco plants.Transgenic research · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We compared the expression of a functional recombinant TMV-specific full-size antibody (rAb29) in both the apoplast and cytosol of tobacco plants and a single chain antibody fragment (scFv29), derived from rAb29, was expressed in the cytosol. Cloned heavy and light chain cDNAs of full-size rAb29, which binds to TMV coat protein monomers, were integrated into the plant expression vector pSS. The full-size rAb29 was expressed in the cytosol and targeted to the apoplast by including the original murine antibody leader sequences. Levels of functional full-size rAb29 expression were high in the apoplast (up to 8.5 micrograms per gram leaf tissue), whereas cytosolic expression was low or at the ELISA detection limit. Sequences of the variable domains of rAb29 light and heavy chain were used to generate the single chain antibody of scFv29, which was expressed in the periplasmic space of E. coli and showed the same binding specificity as full-size rAb29. In addition, scFv29 was functionally expressed in the cytosol of tobacco plants and plant derived scFv29 maintained same binding specificity to TMV-coat protein monomers as rAb29.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.