ArticleThe Biochemical journal2000
Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase.
Article in The Biochemical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 86 citations in OpenAlex.
- GLS1-RNA Polymerase II Axis Mediates Glutamine-Dependent Hepatoprotective Effects on Alcoholic Liver Disease in High-Protein Diets.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Glutaminase inhibition as potential cancer therapeutics: current status and future applications.Journal of enzyme inhibition and medicinal chemistry · 2024Review
- Glutamine addiction in tumor cell: oncogene regulation and clinical treatment.Cell communication and signaling : CCS · 2024Review
- Two Faces of Glutaminase GLS2 in Carcinogenesis.Cancers · 2023Review
- Article
- The 'Achilles Heel' of Metabolism in Renal Cell Carcinoma: Glutaminase Inhibition as a Rational Treatment Strategy.Kidney cancer (Clifton, Va.) · 2019Review
- Liver-enriched Genes are Associated with the Prognosis of Patients with Hepatocellular Carcinoma.Scientific reports · 2018Article
- Glutamate and Brain Glutaminases in Drug Addiction.Neurochemical research · 2017Review
- Tumour-Derived Glutamate: Linking Aberrant Cancer Cell Metabolism to Peripheral Sensory Pain Pathways.Current neuropharmacology · 2017Article
- A tale of two glutaminases: homologous enzymes with distinct roles in tumorigenesis.Future medicinal chemistry · 2017Review
- PEG-PHB-glutaminase nanoparticle inhibits cancer cell proliferation in vitro through glutamine deprivation.In vitro cellular & developmental biology. Animal · 2015Article
- Determination of phosphate-activated glutaminase activity and its kinetics in mouse tissues using metabolic mapping (quantitative enzyme histochemistry).The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2014Article
- Discovery of selective inhibitors of Glutaminase-2, which inhibit mTORC1, activate autophagy and inhibit proliferation in cancer cells.Oncotarget · 2014Article
- ErbB2 activation upregulates glutaminase 1 expression which promotes breast cancer cell proliferation.Journal of cellular biochemistry · 2014Article
- Silencing of GLS and overexpression of GLS2 genes cooperate in decreasing the proliferation and viability of glioblastoma cells.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2014Article
- GLS2 is transcriptionally regulated by p73 and contributes to neuronal differentiation.Cell cycle (Georgetown, Tex.) · 2013Article
- p53 and regulation of tumor metabolism.Journal of carcinogenesis · 2013Review
- Therapeutic strategies impacting cancer cell glutamine metabolism.Future medicinal chemistry · 2013Review
- Dibenzophenanthridines as inhibitors of glutaminase C and cancer cell proliferation.Molecular cancer therapeutics · 2012Article
- Cocaine modulates both glutaminase gene expression and glutaminase activity in the brain of cocaine-sensitized mice.Psychopharmacology · 2012Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphate-activated glutaminase (GA) is overexpressed in certain types of tumour but its exact role in tumour cell growth and proliferation is unknown. Here we describe the isolation of a full-length cDNA clone of human breast cancer ZR75 cells, by a combination of lambdagt10 cDNA library screening and the rapid amplification of cDNA ends ('RACE') technique. The cDNA of human GA is 2408 nt with a 1806-base open reading frame encoding a 602-residue protein with a predicted molecular mass of 66309 Da. The deduced amino acid sequence contains a putative mitochondrial import presequence of 14 residues at the N-terminal end. Heterologous expression and purification in Escherichia coli yielded a product of the expected molecular size that was recognized by using antibodies against the recombinant human GA. Sequence analyses showed that human GA was highly similar to the rat liver enzyme. Northern gel analysis revealed that the gene is present in human liver, brain and pancreas, in which a major transcript of 2.4 kb was demonstrated, but not in kidney, heart, skeletal muscle, lung or placenta. These results strongly suggest that the first human GA cloned, the GA from ZR-75 breast cancer cells, and presumably those from human liver and brain, are liver-type isoenzymes, in sharp contrast with the present view that considers the kidney type as the isoform expressed in all tissues with GA activity, with the exception of postnatal liver.
Indexed as
Identifiers
10620514PMC1220766W2074064379What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.