ArticleBiophysical journal2000
Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study.
Article in Biophysical journal, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 318 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
318 citing papers in PubMed.
- Active and probe-free intracellular rheology via phase-sensitive thermoviscous flows.PNAS nexus · 2026Article
- Laminaran Attenuates NaCl-Induced Cytotoxicity via ROS Scavenging and Prevents Alteration of Cellular Elastic Modulus.Marine drugs · 2026Article
- Optimal design for multi-compartment cell elasticity estimation using AFM and super-resolution imaging.Computer methods and programs in biomedicine · 2026Article
- TheMaterials today. Bio · 2026Review
- Review
- Mechanochemical interactions in cancer cells: The role of substrate stiffness in cell behavior and drug response.PloS one · 2026Article
- Mechanical Environment Afforded by Engineered Hydrogel Critically Regulates Survival of Neural Stem Cells Transplanted in the Injured Spinal Cord via Piezo1-Mediated Mechanotransduction.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Atomic Force Microscopy to Investigate Collagen Fibrils.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Comparison of viscoelastic properties of breast cancer and normal cells using AFM, FLIM and ToF-SIMS techniques.Scientific reports · 2025Article
- A microfluidic rheometer for tumor mechanics and invasion studies.Lab on a chip · 2025Article
- Mechanical Properties of Endothelial Cells: A Key to Physiology, Drug Testing and Nanostructure Interaction.Cells · 2025Review
- A microfluidic rheometer for tumor mechanics and invasion studies.bioRxiv : the preprint server for biology · 2025Article
- Biofilms Exposed: Innovative Imaging and Therapeutic Platforms for Persistent Infections.Antibiotics (Basel, Switzerland) · 2025Review
- Multiplexed Nanoscale Viscoelastic Mapping at Multiple Time Scales of Melanoma Cells as a Label-Free Cancer Biomarker.ACS nano · 2025Article
- Cytochalasins Suppress 3D Migration of ECM-Embedded Tumoroids at Non-Toxic Concentrations.International journal of molecular sciences · 2025Article
- Elastic properties of leukemic cells linked to maturation stage and integrin activation.iScience · 2025Article
- Effects of actin remodeling inhibitors on cellular energy metabolism of a model marine bivalve, the Pacific oyster.The Journal of experimental biology · 2025Article
- Microtubules Disruption Alters the Cellular Structures and Mechanics Depending on Underlying Chemical Cues.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- New Nanobioceramics Based on Hydroxyapatite for Biomedical Applications: Stability and Properties.Nanomaterials (Basel, Switzerland) · 2025Article
- Global alignment and local curvature of microtubules in mouse fibroblasts are robust against perturbations of vimentin and actin.Soft matter · 2025Article
258 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The effect of various drugs affecting the integrity of different components of the cytoskeleton on the elasticity of two fibroblast cell lines was investigated by elasticity measurements with an atomic force microscope (AFM). Disaggregation of actin filaments always resulted in a distinct decrease in the cell's average elastic modulus indicating the crucial importance of the actin network for the mechanical stability of living cells. Disruption or chemical stabilization of microtubules did not affect cell elasticity. For the f-actin-disrupting drugs different mechanisms of drug action were observed. Cytochalasins B and D and Latrunculin A disassembled stress fibers. For Cytochalasin D this was accompanied by an aggregation of actin within the cytosol. Jasplakinolide disaggregated actin filaments but did not disassemble stress fibers. Fibrous structures found in AFM images and elasticity maps of fibroblasts could be identified as stress fibers by correlation of AFM data and fluorescence images.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.