ArticleProceedings of the National Academy of Sciences of the United States of America2000
Nucleotide binding and autophosphorylation of the clock protein KaiC as a circadian timing process of cyanobacteria.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 81 papers.
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Who cites it
81 citing papers in PubMed, 198 citations in OpenAlex.
- From phenomenon to mechanism in the circadian clock of Synechococcus elongatus.Npj biological timing and sleep · 2026Review
- Effects of the signaling molecule cyclic-di-GMP on cyanobacterial circadian rhythm inJournal of bacteriology · 2026Article
- Mutations in the circadian cycle drive adaptive plasticity in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- KaiC family ATPases in the nonheterocystous nitrogen-fixing cyanobacterium Leptolyngbya boryana.Scientific reports · 2024Article
- The inner workings of an ancient biological clock.Trends in biochemical sciences · 2024Review
- Determining subunit-subunit interaction from statistics of cryo-EM images: observation of nearest-neighbor coupling in a circadian clock protein complex.Nature communications · 2023Article
- The increasing role of structural proteomics in cyanobacteria.Essays in biochemistry · 2023Review
- Dimer dissociation is a key energetic event in the fold-switch pathway of KaiB.Biophysical journal · 2022Article
- Involvement of glycogen metabolism in circadian control of UV resistance in cyanobacteria.PLoS genetics · 2020Article
- Damped circadian oscillation in the absence of KaiA in Synechococcus.Nature communications · 2020Article
- Article
- Review
- Cellular Timekeeping: It's Redox o'Clock.Cold Spring Harbor perspectives in biology · 2018Review
- Minimal tool set for a prokaryotic circadian clock.BMC evolutionary biology · 2017Article
- Structural basis of the day-night transition in a bacterial circadian clock.Science (New York, N.Y.) · 2017Article
- A thermodynamically consistent model of the post-translational Kai circadian clock.PLoS computational biology · 2017Article
- Architecture and mechanism of the central gear in an ancient molecular timer.Journal of the Royal Society, Interface · 2017Review
- Systems Biology-Derived Discoveries of Intrinsic Clocks.Frontiers in neurology · 2017Review
- Conversion between two conformational states of KaiC is induced by ATP hydrolysis as a trigger for cyanobacterial circadian oscillation.Scientific reports · 2016Article
- Characterization of the autophosphorylation property of HflX, a ribosome-binding GTPase from Escherichia coli.FEBS open bio · 2016Article
21 more citing papers are in PubMed but not listed here.
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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A negative feedback control of kaiC expression by KaiC protein has been proposed to generate a basic oscillation of the circadian clock in the cyanobacterium Synechococcus sp. PCC 7942. KaiC has two P loops or Walker's motif As, that are potential ATP-/GTP-binding motifs and DXXG motifs conserved in various GTP-binding proteins. Herein, we demonstrate that in vitro KaiC binds ATP and, with lower affinity, GTP. Point mutation by site-directed mutagenesis of P loop 1 completely nullified the circadian rhythm of kaiBC expression and markedly reduced ATP-binding activity. Moreover, KaiC can be autophosphorylated in vitro. These results suggest that the nucleotide-binding activity of KaiC plays important roles in the generation of circadian oscillation in cyanobacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.