ArticleProceedings of the National Academy of Sciences of the United States of America2000
Clustered DNA damages induced in isolated DNA and in human cells by low doses of ionizing radiation.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 152 papers.
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152 citing papers in PubMed.
- Immunogenic Cell Death in Melanoma Induced by Radiotherapy, Photodynamic Therapy, or Non-Thermal Plasma: The Role of Reactive Oxygen and Nitrogen Species.Biomedicines · 2026Review
- Atmospheric Particulate Matter as a Carrier of Pb, Cd, and Ni: From Environmental Transfer and Bioaccessibility to Molecular Toxicity and Predictive Modeling.Journal of xenobiotics · 2026Review
- Immunological Reprogramming by Radiation Therapy: Implications for Precision Cancer Treatment.Immunology · 2026Review
- Inhibition of ATM reverses radioiodine resistance in differentiated thyroid cancer via genotoxic stress amplification.Journal of translational medicine · 2026Article
- Multinomial probability model of radiation induced DSB and non-DSB clusters: tandem and bistranded damage clusters.Scientific reports · 2026Article
- SOHLH2-RAD54L axis induces radioresistance by promoting homologous recombination repair in non-small cell lung cancer.Cell death discovery · 2026Article
- Chlorella-derived extracellular vesicle-based nanogels suppress cGAS-STING for treatment of radiation-induced lung injury.Nature communications · 2026Article
- Adverse Outcome Pathways Applied to Space Radiation Research.Environmental and molecular mutagenesis · 2025Review
- Modeling Clustered DNA Damage by Ionizing Radiation Using Multinomial Damage Probabilities and Energy Imparted Spectra.International journal of molecular sciences · 2024Article
- 8-OxodG: A Potential Biomarker for Chronic Oxidative Stress Induced by High-LET Radiation.DNA · 2024Article
- The role of DNA polymerase I in tolerating single-strand breaks generated at clustered DNA damage in Escherichia coli.Scientific reports · 2024Article
- Radiation Induced Skin Fibrosis (RISF): Opportunity for Angiotensin II-Dependent Intervention.International journal of molecular sciences · 2024Review
- When DNA-damage responses meet innate and adaptive immunity.Cellular and molecular life sciences : CMLS · 2024Review
- Derivation of a comprehensive semi-empirical proton RBE model from published experimental cell survival data collected in the PIDE database.Frontiers in oncology · 2024Article
- Role of Capillaroscopy in Early Diagnosis of Ionizing Radiation Damage in Healthcare Professionals.Medicina (Kaunas, Lithuania) · 2023Article
- The Cellular Response to Complex DNA Damage Induced by Ionising Radiation.International journal of molecular sciences · 2023Review
- Immediate effects of acute Mars mission equivalent doses of SEP and GCR radiation on the murine gastrointestinal system-protective effects of curcumin-loaded nanolipoprotein particles (cNLPs).Frontiers in astronomy and space sciences · 2023Article
- Clustered DNA Damage Patterns after Proton Therapy Beam Irradiation Using Plasmid DNA.International journal of molecular sciences · 2022Article
- On the effectiveness of proton boron fusion therapy (PBFT) at cellular level.Scientific reports · 2022Article
- Transcriptome analysis of human cholangiocytes exposed to carcinogenic 1,2-dichloropropane in the presence of macrophages in vitro.Scientific reports · 2022Article
92 more citing papers are in PubMed but not listed here.
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4 authors.
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Abstract
Clustered DNA damages-two or more closely spaced damages (strand breaks, abasic sites, or oxidized bases) on opposing strands-are suspects as critical lesions producing lethal and mutagenic effects of ionizing radiation. However, as a result of the lack of methods for measuring damage clusters induced by ionizing radiation in genomic DNA, neither the frequencies of their production by physiological doses of radiation, nor their repairability, nor their biological effects are known. On the basis of methods that we developed for quantitating damages in large DNAs, we have devised and validated a way of measuring ionizing radiation-induced clustered lesions in genomic DNA, including DNA from human cells. DNA is treated with an endonuclease that induces a single-strand cleavage at an oxidized base or abasic site. If there are two closely spaced damages on opposing strands, such cleavage will reduce the size of the DNA on a nondenaturing gel. We show that ionizing radiation does induce clustered DNA damages containing abasic sites, oxidized purines, or oxidized pyrimidines. Further, the frequency of each of these cluster classes is comparable to that of frank double-strand breaks; among all complex damages induced by ionizing radiation, double-strand breaks are only about 20%, with other clustered damage constituting some 80%. We also show that even low doses (0.1-1 Gy) of high linear energy transfer ionizing radiation induce clustered damages in human cells.
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