ArticleClinical and diagnostic laboratory immunology2000
Optimization of the weck-Cel collection method for quantitation of cytokines in mucosal secretions.
Article in Clinical and diagnostic laboratory immunology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 53 citations in OpenAlex.
- Effects of the levonorgestrel-releasing intrauterine device on the immune microenvironment of the human cervix and endometrium.American journal of reproductive immunology (New York, N.Y. : 1989) · 2016Trial
- Trial
- Comparison of ophthalmic sponges for measurements of immune markers from cervical secretions.Clinical and diagnostic laboratory immunology · 2004Trial
- Association Between Cervical Inflammatory Mediators and Prevalent Cervical Human Papillomavirus Infection.JCO global oncology · 2024Article
- Pre-analytical sample handling effects on tear fluid protein levels.Scientific reports · 2023Article
- Ascending Reproductive Tract Infection in Pig-Tailed Macaques Inoculated with Mycoplasma genitalium.Infection and immunity · 2022Article
- Critical role of mass spectrometry proteomics in tear biomarker discovery for multifactorial ocular diseases (Review).International journal of molecular medicine · 2021Review
- Cervical Cytokines Associated With Chlamydia trachomatis Susceptibility and Protection.The Journal of infectious diseases · 2019Article
- Menstrual cycle-dependent alterations in glycosylation: a roadmap for defining biomarkers of favorable and unfavorable mucus.Journal of assisted reproduction and genetics · 2019Article
- Lower genital tract cytokine profiles in South African women living with HIV: influence of mucosal sampling.Scientific reports · 2018Article
- Optimising the collection of female genital tract fluid for cytokine analysis in pregnant women.Journal of immunological methods · 2018Article
- Discovery of Blood Transcriptional Endotypes in Women with Pelvic Inflammatory Disease.Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- Absorption of Nasal and Bronchial Fluids: Precision Sampling of the Human Respiratory Mucosa and Laboratory Processing of Samples.Journal of visualized experiments : JoVE · 2018Article
- Determination of cytokine protein levels in oral secretions in patients undergoing radiotherapy for head and neck malignancies.Radiation oncology (London, England) · 2012Article
- Comparison of ophthalmic sponges and extraction buffers for quantifying cytokine profiles in tears using Luminex technology.Molecular vision · 2012Article
- Characterization of the Human Cervical Mucous Proteome.Clinical proteomics · 2010Article
- Immunogenetic correlates of Neisseria gonorrhoeae infection in adolescents.Sexually transmitted diseases · 2008Article
- Determination of cytokine protein levels in cervical mucus samples from young women by a multiplex immunoassay method and assessment of correlates.Clinical and vaccine immunology : CVI · 2008Article
- Article
- Influence of exogenous reproductive hormones on specific antibody production in genital secretions after vaginal vaccination with recombinant cholera toxin B subunit in humans.Clinical and vaccine immunology : CVI · 2006Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Measurement of immune components in mucosal secretions is important for the evaluation of local immunity at the mucosal surfaces. The Weck-Cel ophthalmic sponge provides a method for the collection of these secretions. The sponge absorbs a relatively large volume of material, therefore allowing for quantitation of multiple immune components. Additionally, it provides a method in which the same device may be used to collect specimens from different mucosal sites, such as the genital tract and oral cavity. This sampling technique has successfully been applied for collection and measurement of antibody in oral and genital tract secretions. The purpose of this work was to optimize the extraction of protein from the sponge matrix. Of particular interest was the recovery of cytokines from the sponge. Satisfactory recovery of the cytokines interleukin 1beta (IL-1beta), IL-2, IL-5, IL-12, IL-6, IL-8, IL-10, and granulocyte-macrophage colony-stimulating factor was obtained. However, IL-4 and gamma interferon recovery rates remained low. Using an alteration of the published extraction method, cytokine concentrations were measured in cervical secretions from women using oral contraceptives. The data revealed detectable concentrations of IL-6, IL-10, IL-8, and IL-12 on cycle days 9 and 20. The proposed technique provides an easy, practical, and consistent method for collection of nonconventional body fluids, such as cervicovaginal fluids and saliva, for the assay of immunoglobulins and several cytokines.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.