Evidence map›Paper›PMID 10618269›Full record

ArticleClinical and diagnostic laboratory immunology2000

Opsonization and phagocytosis of Plasmodium falciparum merozoites measured by flow cytometry.

L M Kumaratilake, A Ferrante

Open access · diamondAbstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Clinical and diagnostic laboratory immunology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.

0numbers the graph read from it
0cells of the map it votes in
22citing papers in PubMed
0.8field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

22 citing papers in PubMed, 47 citations in OpenAlex.

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  8. Accelerator or Brake: Immune Regulators in Malaria.Frontiers in cellular and infection microbiology · 2020
    Review
  9. Article
  10. Review
  11. Neutrophils and Malaria.Frontiers in immunology · 2018
    Review
  12. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

L M KumaratilakeDepartment of Immunopathology, Women's and Children's Hospital, Adelaide, South Australia 5006, Australia. lkumarat@medicine.adelaide.edu.au
A Ferrante
University of Adelaide · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A flow cytometric phagocytosis assay was established to investigate the role of anti-merozoite antibody, complement, and cytokines on the phagocytosis of Plasmodium falciparum merozoites by human neutrophils. This assay involved allowing fluorescein isothiocyanate-labeled merozoites to interact with phagocytes and analysis of the cells on a FACScan with Lysis II software. To differentiate the proportion of neutrophil surface-bound merozoites from the merozoites ingested by neutrophils, the fluorescence of bound merozoites was quenched by adding trypan blue. The data showed that sera from malaria-immune individuals in the Solomon Islands and Papua New Guinea promoted merozoite engulfment by neutrophils. The cytokines tumor necrosis factor alpha, gamma interferon, granulocyte-macrophage colony-stimulating factor, and interleukin-1beta significantly increased the amount and the rate of merozoite phagocytosis by neutrophils. Optimum merozoite phagocytosis occurred when both cytokines and anti-malarial antibody were present.

Indexed as

Flow CytometryAnimalsAntibodiesCytokinesFluorescein-5-isothiocyanateFluorescenceHumansInterferon-gammaMelanesiaMerozoite Surface Protein 1NeutrophilsOpsonin ProteinsPapua New GuineaPhagocytesPhagocytosisPlasmodium falciparumAntibodiesCytokinesFluorescein-5-isothiocyanateInterferon-gammaMerozoite Surface Protein 1Opsonin ProteinsRecombinant ProteinsTrypan Blue

Identifiers

PMID10618269
PMCPMC95814
OpenAlexW2101158818

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.