ArticleApplied and environmental microbiology2000
Quantification of different Eubacterium spp. in human fecal samples with species-specific 16S rRNA-targeted oligonucleotide probes.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Breastfeeding Affects Concentration of Faecal Short Chain Fatty Acids During the First Year of Life: Results of the Systematic Review and Meta-Analysis.Frontiers in nutrition · 2022Pooled it
- Meta-analysis of effects of exclusive breastfeeding on infant gut microbiota across populations.Nature communications · 2018Pooled it
- Trial
- Eggerthella lenta disrupts the gut vascular barrier to drive colorectal cancer liver metastasis.Cell discovery · 2026Article
- Rainfall-driven dynamics of faecal pollution in river bathing sites: coupling hydrological events, and DNA-assisted tracking to improve pollution management in recreational waters.Frontiers in microbiology · 2026Article
- Advancements in analytical methods for studying the human gut microbiome.Journal of biological methods · 2025Review
- Identification of inulin-responsive bacteria in the gut microbiota via multi-modal activity-based sorting.Nature communications · 2023Article
- Immunometabolic Analysis ofFrontiers in cellular and infection microbiology · 2021Article
- Analysis of the mouse gut microbiome using full-length 16S rRNA amplicon sequencing.Scientific reports · 2016Article
- Capturing One of the Human Gut Microbiome's Most Wanted: Reconstructing the Genome of a Novel Butyrate-Producing, Clostridial Scavenger from Metagenomic Sequence Data.Frontiers in microbiology · 2016Article
- Quantification of Slackia and Eggerthella spp. in Human Feces and Adhesion of Representatives Strains to Caco-2 Cells.Frontiers in microbiology · 2016Article
- Quantifying the metabolic activities of human-associated microbial communities across multiple ecological scales.FEMS microbiology reviews · 2013Review
- Article
- Microarray analysis and barcoded pyrosequencing provide consistent microbial profiles depending on the source of human intestinal samples.Applied and environmental microbiology · 2011Article
- Article
- Bifidobacterial lipoglycan as a new cause for false-positive platelia Aspergillus enzyme-linked immunosorbent assay reactivity.Journal of clinical microbiology · 2005Article
- Colonic microbiota signatures across five northern European countries.Applied and environmental microbiology · 2005Article
- Identification of bacterial populations in dairy wastewaters by use of 16S rRNA gene sequences and other genetic markers.Applied and environmental microbiology · 2004Article
- New real-time quantitative PCR procedure for quantification of bifidobacteria in human fecal samples.Applied and environmental microbiology · 2004Article
- Anaerobic degradation of flavonoids by Clostridium orbiscindens.Applied and environmental microbiology · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Species-specific 16S rRNA-targeted, Cy3 (indocarbocyanine)-labeled oligonucleotide probes were designed and validated to quantify different Eubacterium species in human fecal samples. Probes were directed at Eubacterium barkeri, E. biforme, E. contortum, E. cylindroides (two probes), E. dolichum, E. hadrum, E. lentum, E. limosum, E. moniliforme, and E. ventriosum. The specificity of the probes was tested with the type strains and a range of common intestinal bacteria. With one exception, none of the probes showed cross-hybridization under stringent conditions. The species-specific probes were applied to fecal samples obtained from 12 healthy volunteers. E. biforme, E. cylindroides, E. hadrum, E. lentum, and E. ventriosum could be determined. All other Eubacterium species for which probes had been designed were under the detection limit of 10(7) cells g (dry weight) of feces(-1). The cell counts obtained are essentially in accordance with the literature data, which are based on colony counts. This shows that whole-cell in situ hybridization with species-specific probes is a valuable tool for the enumeration of Eubacterium species in feces.
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Registered trials
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