ArticleApplied and environmental microbiology2000
Sequence analysis of the cryptic plasmid pMG101 from Rhodopseudomonas palustris and construction of stable cloning vectors.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 42 citations in OpenAlex.
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- Integrated roles of BclA and DD-carboxypeptidase 1 in Bradyrhizobium differentiation within NCR-producing and NCR-lacking root nodules.Scientific reports · 2017Article
- Metabolic engineering ofBiotechnology for biofuels · 2017Article
- Metabolic engineering of Rhodopseudomonas palustris for squalene production.Journal of industrial microbiology & biotechnology · 2016Article
- Improving coenzyme Q8 production in Escherichia coli employing multiple strategies.Journal of industrial microbiology & biotechnology · 2014Article
- Photosynthetic Bradyrhizobium sp. strain ORS285 is capable of forming nitrogen-fixing root nodules on soybeans (Glycine max).Applied and environmental microbiology · 2013Article
- Structure-guided expansion of the substrate range of methylmalonyl coenzyme A synthetase (MatB) of Rhodopseudomonas palustris.Applied and environmental microbiology · 2012Article
- Novel carotenoid-based biosensor for simple visual detection of arsenite: characterization and preliminary evaluation for environmental application.Applied and environmental microbiology · 2008Article
- Molecular dissection of a bifidobacterial replicon.Applied and environmental microbiology · 2007Article
- Isolation and molecular characterization of pMG160, a mobilizable cryptic plasmid from Rhodobacter blasticus.Applied and environmental microbiology · 2003Article
- Complete nucleotide sequence of a 43-kilobase genomic island associated with the multidrug resistance region of Salmonella enterica serovar Typhimurium DT104 and its identification in phage type DT120 and serovar Agona.Journal of bacteriology · 2001Article
- Divergent mechanisms of 5' 23S rRNA IVS processing in the alpha-proteobacteria.Nucleic acids research · 2000Article
- Complete nucleotide sequence of ubiquitous plasmid pEA29 from Erwinia amylovora strain Ea88: gene organization and intraspecies variation.Applied and environmental microbiology · 2000Article
- Phenotypic and physiological changes in the phototrophic purple nonsulfur bacterium Rhodopseudomonas palustris transformed with the binary vector pGA482 carrying the cytokinin biosynthesis gene ipt.Doklady. Biochemistry and biophysicsArticle
- Direct transition of pGA482:ipt plasmid bearing the cytokinin biosynthesis gene into the cells of phototrophic purple bacteria Rhodobacter sphaeroides and Rhodopseudomonas palustris by electroporation.Doklady. Biochemistry and biophysicsArticle
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4 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A 15-kb cryptic plasmid was obtained from a natural isolate of Rhodopseudomonas palustris. The plasmid, designated pMG101, was able to replicate in R. palustris and in closely related strains of Bradyrhizobium japonicum and phototrophic Bradyrhizobium species. However, it was unable to replicate in the purple nonsulfur bacterium Rhodobacter sphaeroides and in Rhizobium species. The replication region of pMG101 was localized to a 3.0-kb SalI-XhoI fragment, and this fragment was stably maintained in R. palustris for over 100 generations in the absence of selection. The complete nucleotide sequence of this fragment revealed two open reading frames (ORFs), ORF1 and ORF2. The deduced amino acid sequence of ORF1 is similar to sequences of Par proteins, which mediate plasmid stability from certain plasmids, while ORF2 was identified as a putative rep gene, coding for an initiator of plasmid replication, based on homology with the Rep proteins of several other plasmids. The function of these sequences was studied by deletion mapping and gene disruptions of ORF1 and ORF2. pMG101-based Escherichia coli-R. palustris shuttle cloning vectors pMG103 and pMG105 were constructed and were stably maintained in R. palustris growing under nonselective conditions. The ability of plasmid pMG101 to replicate in R. palustris and its close phylogenetic relatives should enable broad application of these vectors within this group of alpha-proteobacteria.
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