ArticleApplied and environmental microbiology2000
Molecular and functional analyses of the metC gene of Lactococcus lactis, encoding cystathionine beta-lyase.
Article in Applied and environmental microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 81 citations in OpenAlex.
- Article
- A Perspective Study of Koumiss Microbiome by Metagenomics Analysis Based on Single-Cell Amplification Technique.Frontiers in microbiology · 2017Article
- Stress Physiology of Lactic Acid Bacteria.Microbiology and molecular biology reviews : MMBR · 2016Review
- Article
- Fermentation-induced variation in heat and oxidative stress phenotypes of Lactococcus lactis MG1363 reveals transcriptome signatures for robustness.Microbial cell factories · 2014Article
- Computational analysis of cysteine and methionine metabolism and its regulation in dairy starter and related bacteria.Journal of bacteriology · 2012Article
- The genome sequence of Geobacter metallireducens: features of metabolism, physiology and regulation common and dissimilar to Geobacter sulfurreducens.BMC microbiology · 2009Article
- Heterologous production of methionine-gamma-lyase from Brevibacterium linens in Lactococcus lactis and formation of volatile sulfur compounds.Applied and environmental microbiology · 2009Article
- Comparative genomics of enzymes in flavor-forming pathways from amino acids in lactic acid bacteria.Applied and environmental microbiology · 2008Review
- Cloning and characterization of two Lactobacillus casei genes encoding a cystathionine lyase.Applied and environmental microbiology · 2008Article
- Role of cystathionine beta-lyase in catabolism of amino acids to sulfur volatiles by genetic variants of Lactobacillus helveticus CNRZ 32.Applied and environmental microbiology · 2007Article
- Construction of plasmid vector for expression of bacteriocin N15-encoding gene and effect of engineered bacteria on Enterococcus faecalis.Current microbiology · 2007Article
- YtjE from Lactococcus lactis IL1403 Is a C-S lyase with alpha, gamma-elimination activity toward methionine.Applied and environmental microbiology · 2006Article
- Sulfur amino acid metabolism and its control in Lactococcus lactis IL1403.Journal of bacteriology · 2005Article
- Identification and functional analysis of the gene encoding methionine-gamma-lyase in Brevibacterium linens.Applied and environmental microbiology · 2004Article
- Ability of thermophilic lactic acid bacteria to produce aroma compounds from amino acids.Applied and environmental microbiology · 2004Article
- Regulation of the metC-cysK operon, involved in sulfur metabolism in Lactococcus lactis.Journal of bacteriology · 2002Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The enzymatic degradation of amino acids in cheese is believed to generate aroma compounds and therefore to be essential for flavor development. Cystathionine beta-lyase (CBL) can convert cystathionine to homocysteine but is also able to catalyze an alpha, gamma elimination. With methionine as a substrate, it produces volatile sulfur compounds which are important for flavor formation in Gouda cheese. The metC gene, which encodes CBL, was cloned from the Lactococcus lactis model strain MG1363 and from strain B78, isolated from a cheese starter culture and known to have a high capacity to produce volatile compounds. The metC gene was found to be cotranscribed with a downstream cysK gene, which encodes a putative cysteine synthase. The MetC proteins of both strains were overproduced in strain MG1363 with the NICE (nisin-controlled expression) system, resulting in a >25-fold increase in cystathionine lyase activity. A disruption of the metC gene was achieved in strain MG1363. Determination of enzymatic activities in the overproducing and knockout strains revealed that MetC is essential for the degradation of cystathionine but that at least one lyase other than CBL contributes to methionine degradation via alpha, gamma elimination to form volatile aroma compounds.
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