ArticleJournal of clinical microbiology2000
Optimized PCR using patient blood samples for diagnosis and follow-up of visceral Leishmaniasis, with special reference to AIDS patients.
Article in Journal of clinical microbiology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 3 of them syntheses that pooled it.
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Who cites it
45 citing papers in PubMed, 3 syntheses or guidelines pooled it.
- Global Landscape of Molecular and Immunological Diagnostic Tests for Human Leishmaniasis: A Systematic Review and Meta-Analysis.Pathogens (Basel, Switzerland) · 2025Pooled it
- The diagnostic accuracy of serologic and molecular methods for detecting visceral leishmaniasis in HIV infected patients: meta-analysis.PLoS neglected tropical diseases · 2012Pooled it
- Predictors of visceral leishmaniasis relapse in HIV-infected patients: a systematic review.PLoS neglected tropical diseases · 2011Pooled it
- Blood Parasite Load as an Early Marker to Predict Treatment Response in Visceral Leishmaniasis in Eastern Africa.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Trial
- The utility of pathogen inactivation technology: a real-life example of Leishmania infantum inactivation in platelets from a donor with an asymptomatic infection.Blood transfusion = Trasfusione del sangue · 2012Trial
- Evaluation of PCR for diagnosis of Indian kala-azar and assessment of cure.Journal of clinical microbiology · 2005Trial
- Leishmania blood parasite dynamics during and after treatment of visceral leishmaniasis in Eastern Africa: A pharmacokinetic-pharmacodynamic model.PLoS neglected tropical diseases · 2024Article
- Molecular Tracking of theFrontiers in cellular and infection microbiology · 2021Review
- Frequency of Leishmania spp. infection among HIV-infected patients living in an urban area in Brazil: a cross-sectional study.BMC infectious diseases · 2020Article
- Dermotropic Leishmania donovani in Sri Lanka: visceralizing potential in clinical and preclinical studies.Parasitology · 2018Article
- Performance of two immunochromatographic tests for diagnosis of visceral leishmaniasis in patients coinfected with HIV.Parasitology research · 2018Article
- Development and Validation of a PCR-ELISA for the Diagnosis of Symptomatic and Asymptomatic Infection byJournal of tropical medicine · 2017Article
- Serological and molecular tools to diagnose visceral leishmaniasis: 2-years' experience of a single center in Northern Italy.PloS one · 2017Article
- Synthetic Peptides as Potential Antigens for Cutaneous Leishmaniosis Diagnosis.Journal of immunology research · 2017Article
- Screening for subclinical Leishmania infection in HIV-infected patients living in eastern Spain.Pathogens and global health · 2014Article
- Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil.BMC infectious diseases · 2014Article
- [Pericardial tamponade revealing systemic lupus erythematosus during the course of atypical visceral leishmaniasis].The Pan African medical journal · 2014Article
- Ultrasensitive real-time PCR for the clinical management of visceral leishmaniasis in HIV-Infected patients.The American journal of tropical medicine and hygiene · 2013Article
- Quantification of Leishmania infantum kinetoplast DNA for monitoring the response to meglumine antimoniate therapy in visceral leishmaniasis.The American journal of tropical medicine and hygiene · 2013Article
- Evaluation of toxicity & therapeutic efficacy of a new liposomal formulation of amphotericin B in a mouse model.The Indian journal of medical research · 2013Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a highly sensitive PCR method that enables the diagnosis and posttherapeutic follow-up of visceral leishmaniasis with patient blood. The PCR assay was thoroughly optimized by successive procedural refinements to increase its sensitivity and specificity. It was compared to in vitro cultivation as well as to direct examination of bone marrow and to serology. Two hundred thirty-seven patients presenting with clinical signs compatible with visceral leishmaniasis were included in the study. Thirty-six were diagnosed as having Mediterranean visceral leishmaniasis (MVL). Twenty-three of them, including 19 AIDS patients, were monitored during and after treatment over a period from 2 weeks to 3 years. Our PCR assay proved more sensitive than in vitro cultivation, direct examination, and serology for all patients. It is simple and can be adapted to routine hospital diagnostic procedures. For the primary diagnosis of MVL, the sensitivity of PCR versus that of cultivation was 97 versus 55% with peripheral blood and 100 versus 81% with bone marrow samples. Regarding posttherapeutic follow-up, overall, 48% of positive samples were detected by PCR only. Seven patients presented with a clinical relapse during the study; six relapses were detected at first by PCR only, sometimes a few weeks before the reappearance of signs or symptoms. We conclude that an optimized and well-mastered PCR assay with a peripheral blood sample is sufficient to provide a secure diagnosis for all immunocompromised patients and most immunocompetent patients. We also suggest systematic posttherapeutic monitoring by PCR with peripheral blood for immunocompromised patients.
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