ArticleThe Journal of cell biology1999
Intranuclear anchoring of repetitive DNA sequences: centromeres, telomeres, and ribosomal DNA.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Stress granules dynamics: benefits in cancer.BMB reports · 2022Review
- Nuclear envelope tethering inhibits the formation of ALT-associated PML bodies in ALT cells.Aging · 2021Article
- Ribosomal DNA and the Nucleolus as Keystones of Nuclear Architecture, Organization, and Function.Trends in genetics : TIG · 2019Review
- Vitamin D Deficiency: Effects on Oxidative Stress, Epigenetics, Gene Regulation, and Aging.Biology · 2019Review
- Nucleolus and chromatin.Histochemistry and cell biology · 2018Review
- Article
- Dynamics of telomeres and promyelocytic leukemia nuclear bodies in a telomerase-negative human cell line.Molecular biology of the cell · 2009Article
- Chromatin tethering effects of hNopp140 are involved in the spatial organization of nucleolus and the rRNA gene transcription.Journal of biomedical science · 2008Article
- The very long telomeres in Sorex granarius (Soricidae, Eulipothyphla) contain ribosomal DNA.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2007Article
- Cell cycle-dependent recruitment of telomerase RNA and Cajal bodies to human telomeres.Molecular biology of the cell · 2006Article
- The genome and the nucleus: a marriage made by evolution. Genome organisation and nuclear architecture.Chromosoma · 2005Review
- The 4q subtelomere harboring the FSHD locus is specifically anchored with peripheral heterochromatin unlike most human telomeres.The Journal of cell biology · 2004Article
- Visualizing telomere dynamics in living mammalian cells using PNA probes.The EMBO journal · 2003Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Centromeres, telomeres, and ribosomal gene clusters consist of repetitive DNA sequences. To assess their contributions to the spatial organization of the interphase genome, their interactions with the nucleoskeleton were examined in quiescent and activated human lymphocytes. The nucleoskeletons were prepared using "physiological" conditions. The resulting structures were probed for specific DNA sequences of centromeres, telomeres, and ribosomal genes by in situ hybridization; the electroeluted DNA fractions were examined by blot hybridization. In both nonstimulated and stimulated lymphocytes, centromeric alpha-satellite repeats were almost exclusively found in the eluted fraction, while telomeric sequences remained attached to the nucleoskeleton. Ribosomal genes showed a transcription-dependent attachment pattern: in unstimulated lymphocytes, transcriptionally inactive ribosomal genes located outside the nucleolus were eluted completely. When comparing transcription unit and intergenic spacer, significantly more of the intergenic spacer was removed. In activated lymphocytes, considerable but similar amounts of both rDNA fragments were eluted. The results demonstrate that: (a) the various repetitive DNA sequences differ significantly in their intranuclear anchoring, (b) telomeric rather than centromeric DNA sequences form stable attachments to the nucleoskeleton, and (c) different attachment mechanisms might be responsible for the interaction of ribosomal genes with the nucleoskeleton.
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