ArticleJournal of bacteriology2000
Elongation factor Tu and DnaK are transferred from the cytoplasm to the periplasm of Escherichia coli during osmotic downshock presumably via the mechanosensitive channel mscL.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
45 citing papers in PubMed, 103 citations in OpenAlex.
- Salinity-driven niche differentiation within the aquatic Luna-1 subcluster.ISME communications · 2025Article
- Biological characteristics of mechanosensitive channels MscS and MscL inJournal of bacteriology · 2024Article
- Aberrant Topologies of Bacterial Membrane Proteins Revealed by High Sensitivity Fluorescence Labelling.Journal of molecular biology · 2024Article
- Article
- Life with Bacterial Mechanosensitive Channels, from Discovery to Physiology to Pharmacological Target.Microbiology and molecular biology reviews : MMBR · 2020Review
- Novel MscL agonists that allow multiple antibiotics cytoplasmic access activate the channel through a common binding site.PloS one · 2020Article
- An agonist of the MscL channel affects multiple bacterial species and increases membrane permeability and potency of common antibiotics.Molecular microbiology · 2019Article
- Mechanism of action of the moonlighting protein EfTu as a Substance P sensor in Bacillus cereus.Scientific reports · 2019Article
- Host Peptidic Hormones Affecting Bacterial Biofilm Formation and Virulence.Journal of innate immunity · 2019Review
- Effects of Low Intensity Focused Ultrasound on Liposomes Containing Channel proteins.Scientific reports · 2018Article
- Antidromic neurogenic activity and cutaneous bacterial flora.Seminars in immunopathology · 2018Review
- Translation Stress Positively Regulates MscL-Dependent Excretion of Cytoplasmic Proteins.mBio · 2018Article
- Critical role of quorum sensing-dependent glutamate metabolism in homeostatic osmolality and outer membrane vesiculation in Burkholderia glumae.Scientific reports · 2017Article
- Differential Proteome Between Patient-Related and Non-related Environmental Isolates of Legionella pneumophila.Current microbiology · 2017Article
- Substance P and Calcitonin Gene-Related Peptide: Key Regulators of Cutaneous Microbiota Homeostasis.Frontiers in endocrinology · 2017Review
- Skin-bacteria communication: Involvement of the neurohormone Calcitonin Gene Related Peptide (CGRP) in the regulation of Staphylococcus epidermidis virulence.Scientific reports · 2016Article
- Hyperconcentrated Sweet Whey, a New Culture Medium That Enhances Propionibacterium freudenreichii Stress Tolerance.Applied and environmental microbiology · 2016Article
- A Periplasmic Complex of the Nitrite Reductase NirS, the Chaperone DnaK, and the Flagellum Protein FliC Is Essential for Flagellum Assembly and Motility in Pseudomonas aeruginosa.Journal of bacteriology · 2015Article
- Inducible release of particulates from liposomes using the mechanosensitive channel of large conductance and L-α-lysophosphatidylcholine.European biophysics journal : EBJ · 2015Article
- Mealybugs nested endosymbiosis: going into the 'matryoshka' system in Planococcus citri in depth.BMC microbiology · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Upon osmotic downshock, a few cytoplasmic proteins, including thioredoxin, elongation factor Tu (EF-Tu), and DnaK, are released from Tris-EDTA-treated Escherichia coli cells by an unknown mechanism. We have shown previously that deletion of mscL, the gene coding for the mechanosensitive channel of the plasma membrane with the highest conductance, prevents the release of thioredoxin. We confirm and extend the implication of MscL in this process by showing that the release of EF-Tu and DnaK is severely impaired in MscL-deficient strains. Release of these proteins is not observed in the absence of a Tris-EDTA treatment which disrupts the outer membrane, indicating that, in intact cells, they are transferred to the periplasm upon shock, presumably through the MscL channel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.