ArticleJournal of bacteriology2000
Role of HrcA and CIRCE in the heat shock regulatory network of Bradyrhizobium japonicum.
Article in Journal of bacteriology, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 56 citations in OpenAlex.
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- In-Depth Analysis of an Obligate AnaerobeAnimals : an open access journal from MDPI · 2022Article
- EfgA is a conserved formaldehyde sensor that leads to bacterial growth arrest in response to elevated formaldehyde.PLoS biology · 2021Article
- Analysis of temporal gene regulation of Listeria monocytogenes revealed distinct regulatory response modes after exposure to high pressure processing.BMC genomics · 2021Article
- Computational Analysis of the Hypothetical Protein P9303_05031 from Marine Cyanobacterium Prochlorococcus Marinus MIT 9303.Journal of integrative bioinformatics · 2020Article
- Regulatory (pan-)genome of an obligate intracellular pathogen in the PVC superphylum.The ISME journal · 2016Article
- Intra-ChIP: studying gene regulation in an intracellular pathogen.Current genetics · 2016Review
- Transcriptional regulation of the Chlamydia heat shock stress response in an intracellular infection.Molecular microbiology · 2015Article
- Article
- Dual RpoH sigma factors and transcriptional plasticity in a symbiotic bacterium.Journal of bacteriology · 2012Article
- A Chlamydia-specific C-terminal region of the stress response regulator HrcA modulates its repressor activity.Journal of bacteriology · 2011Article
- Characterization of a unique ClpB protein of Mycoplasma pneumoniae and its impact on growth.Infection and immunity · 2008Article
- Global consequences of phosphatidylcholine reduction in Bradyrhizobium japonicum.Molecular genetics and genomics : MGG · 2008Article
- Multiple phospholipid N-methyltransferases with distinct substrate specificities are encoded in Bradyrhizobium japonicum.Journal of bacteriology · 2008Article
- Replicon-specific regulation of small heat shock genes in Agrobacterium tumefaciens.Journal of bacteriology · 2004Article
- Functional analysis of the heat shock regulator HrcA of Chlamydia trachomatis.Journal of bacteriology · 2002Article
- A mRNA-based thermosensor controls expression of rhizobial heat shock genes.Nucleic acids research · 2001Article
- Renaturation of Bacillus thermoglucosidasius HrcA repressor by DNA and thermostability of the HrcA-DNA complex in vitro.Journal of bacteriology · 2001Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A large number of bacteria regulate chaperone gene expression by the CIRCE-HrcA system in which a DNA element called CIRCE serves as binding site for the repressor protein HrcA under non-heat-shock conditions. We have cloned the two consecutive genes hrcA and grpE of Bradyrhizobium japonicum by using a complementation approach that screened for GrpE function. In vivo and in vitro transcript mapping demonstrated that both genes are transcribed separately from RpoH (sigma(32))-dependent promoters. To investigate the supposed negative regulatory function of HrcA, we compared the expression of putative target genes in the wild type with that in an hrcA mutant. Transcription of the CIRCE-associated chaperonin operons groESL(4) and groESL(5), as well as the beta-galactosidase activity derived from corresponding groE-lacZ fusions, was strongly elevated in the hrcA mutant even at physiological temperatures. Expression of other heat shock regulons (RpoH or ROSE dependent) was not affected. To study the activity of HrcA in vitro, we purified a histidine-tagged version of the protein under nondenaturing conditions. Specific binding to the CIRCE element was obtained with a soluble fraction of HrcA in gel retardation experiments.
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