ArticleGenome research1999
Expression profiling by iAFLP: A PCR-based method for genome-wide gene expression profiling.
Article in Genome research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 37 citations in OpenAlex.
- Large-scale tag/PCR-based gene expression profiling.World journal of microbiology & biotechnology · 2014Review
- PCDq: human protein complex database with quality index which summarizes different levels of evidences of protein complexes predicted from h-invitational protein-protein interactions integrative dataset.BMC systems biology · 2012Article
- Characteristics and clustering of human ribosomal protein genes.BMC genomics · 2006Article
- The Human Anatomic Gene Expression Library (H-ANGEL), the H-Inv integrative display of human gene expression across disparate technologies and platforms.Nucleic acids research · 2005Article
- Sequence comparison of human and mouse genes reveals a homologous block structure in the promoter regions.Genome research · 2004Article
- Integrative annotation of 21,037 human genes validated by full-length cDNA clones.PLoS biology · 2004Article
- Identification of water-deficit responsive genes in maritime pine (Pinus pinaster Ait.) roots.Plant molecular biology · 2003Article
- Identification and characterization of the potential promoter regions of 1031 kinds of human genes.Genome research · 2001Article
- BodyMap incorporated PCR-based expression profiling data and a gene ranking system.Nucleic acids research · 2001Article
- BodyMap: a collection of 3' ESTs for analysis of human gene expression information.Genome research · 2000Article
- Current awareness.Yeast (Chichester, England) · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The availability of comprehensive sets of genes has prompted the researchers to carry out systematic collection of gene expression data. RT-PCR has the highest specificity and sensitivity for transcript detection among all available methods. Low throughput, especially when quantitative data are desired, has precluded RT-PCR from genome-wide application. Here we report a PCR-based expression profiling method, introduced amplified fragment length polymorphism (iAFLP), that has the same specificity and sensitivity as RT-PCR and a throughput level comparable to that of DNA-microarray hybridization. In this method, restricted ends of total cDNAs from six sources were ligated with adaptors having various length of short insertions to a common sequence (polymorphic adaptors). Amplification of a pool of these differentially adapted cDNAs with a gene-specific primer and an adaptor primer allows us to quantitate the abundance of any transcript in six mRNA sources. Using three different primer colors this technique allows quantitation of expression of 864 genes across six different sources per day with a single autosequencer, which is comparable to the throughput of microarray analysis in terms of number of genes x number of sources.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.