ArticleProceedings of the National Academy of Sciences of the United States of America1999
Phospholipase C-linked receptors regulate the ATP-sensitive potassium channel by means of phosphatidylinositol 4,5-bisphosphate metabolism.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 88 citations in OpenAlex.
- Methods to study phosphoinositide regulation of ion channels.Methods in enzymology · 2021Article
- Palmitoylation of the KProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Intramolecular electrostatic interactions contribute to phospholipase Cβ3 autoinhibition.Cellular signalling · 2019Article
- Endothelial FAM3A positively regulates post-ischaemic angiogenesis.EBioMedicine · 2019Article
- A biomimetic chiral-driven ionic gate constructed by pillar[6]arene-based host-guest systems.Nature communications · 2018Article
- Running out of time: the decline of channel activity and nucleotide activation in adenosine triphosphate-sensitive K-channels.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2016Review
- Pancreatic Beta Cell G-Protein Coupled Receptors and Second Messenger Interactions: A Systems Biology Computational Analysis.PloS one · 2016Article
- Ca2+ Influx through Store-operated Calcium Channels Replenishes the Functional Phosphatidylinositol 4,5-Bisphosphate Pool Used by Cysteinyl Leukotriene Type I Receptors.The Journal of biological chemistry · 2015Article
- Phosphoinositide control of membrane protein function: a frontier led by studies on ion channels.Annual review of physiology · 2015Review
- Channelopathies linked to plasma membrane phosphoinositides.Pflugers Archiv : European journal of physiology · 2010Review
- Muscle KATP channels: recent insights to energy sensing and myoprotection.Physiological reviews · 2010Review
- The Gq/G11-mediated signaling pathway is critical for autocrine potentiation of insulin secretion in mice.The Journal of clinical investigation · 2010Article
- Noble gas xenon is a novel adenosine triphosphate-sensitive potassium channel opener.Anesthesiology · 2010Article
- Cardiac sarcolemmal K(ATP) channels: Latest twists in a questing tale!Journal of molecular and cellular cardiology · 2010Review
- New properties of mitochondrial ATP-regulated potassium channels.Journal of bioenergetics and biomembranes · 2008Article
- PIP2 PIP2 hooray for maxi K+.The Journal of general physiology · 2008Article
- Phosphatidylinositol 4,5-bisphosphate interactions with the HERG K(+) channel.Pflugers Archiv : European journal of physiology · 2007Review
- Cytoplasmic accumulation of long-chain coenzyme A esters activates KATP and inhibits Kir2.1 channels.The Journal of physiology · 2006Article
- Angiotensin II regulates neuronal excitability via phosphatidylinositol 4,5-bisphosphate-dependent modulation of Kv7 (M-type) K+ channels.The Journal of physiology · 2006Article
- Differential phosphoinositide binding to components of the G protein-gated K+ channel.The Journal of membrane biology · 2006Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In the COS7 cells transfected with cDNAs of the Kir6.2, SUR2A, and M(1) muscarinic receptors, we activated the ATP-sensitive potassium (K(ATP)) channel with a K(+) channel opener and recorded the whole-cell K(ATP) current. The K(ATP) current was reversibly inhibited by the stimulation of the M(1) receptor, which is linked to phospholipase C (PLC) by the G(q) protein. The receptor-mediated inhibition was observed even when protein kinase C (PKC) was inhibited by H-7 or by chelating intracellular Ca(2+) with 10 mM 1, 2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetate (BAPTA) included in the pipette solution. However, the receptor-mediated inhibition was blocked by U-73122, a PLC inhibitor. M(1)-receptor stimulation failed to inhibit the K(ATP) current activated by the injection of exogenous phosphatidylinositol 4,5-bisphosphate (PIP(2)) through the whole-cell patch pipette. The receptor-mediated inhibition became irreversible when the replenishment of PIP(2) was blocked by wortmannin (an inhibitor of phosphatidylinositol kinases), or by including adenosine 5'-[beta,gamma-imido]triphosphate (AMPPNP, a nonhydrolyzable ATP analogue) in the pipette solution. In inside-out patch experiments, the ATP sensitivity of the K(ATP) channel was significantly higher when the M(1) receptor in the patch membrane was stimulated by acetylcholine. The stimulatory effect of pinacidil was also attenuated under this condition. We postulate that stimulation of PLC-linked receptors inhibited the K(ATP) channel by increasing the ATP sensitivity, not through PKC activation, but most probably through changing PIP(2) levels.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.