ArticleProceedings of the National Academy of Sciences of the United States of America1999
The 65-kDa carrot microtubule-associated protein forms regularly arranged filamentous cross-bridges between microtubules.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 197 citations in OpenAlex.
- Cytoskeletal control of development and environmental adaptation in plants.Science China. Life sciences · 2026Review
- PP6 phosphatase and Elongator contribute to kinesin 5-dependent spindle assembly by controlling microtubule regulators levels.PLoS genetics · 2025Article
- The N-terminal domain of COMPANION OF CELLULOSE SYNTHASE1 promotes microtubule array formation in Arabidopsis.Plant physiology · 2025Article
- Probing stress-regulated ordering of the plant cortical microtubule array via a computational approach.BMC plant biology · 2023Article
- Intracellular infection by symbiotic bacteria requires the mitotic kinase AURORA1.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Cell biology of primary cell wall synthesis in plants.The Plant cell · 2022Review
- A rich and bountiful harvest: Key discoveries in plant cell biology.The Plant cell · 2022Review
- Kinesin-14 motors drive a right-handed helical motion of antiparallel microtubules around each other.Nature communications · 2020Article
- Complementary Superresolution Visualization of Composite Plant Microtubule Organization and Dynamics.Frontiers in plant science · 2020Article
- Phosphorylation of Plant Microtubule-Associated Proteins During Cell Division.Frontiers in plant science · 2019Review
- Geometry of antiparallel microtubule bundles regulates relative sliding and stalling by PRC1 and Kif4A.eLife · 2018Article
- A lumenal interrupted helix in human sperm tail microtubules.Scientific reports · 2018Article
- Phragmoplast microtubule dynamics - a game of zones.Journal of cell science · 2018Review
- Proteins immunologically related to MAP65-1 accumulate and localize differentially during bud development in Vitis vinifera L.Protoplasma · 2017Article
- Phosphorylation of MAP65-1 by Arabidopsis Aurora Kinases Is Required for Efficient Cell Cycle Progression.Plant physiology · 2017Article
- Effects of high temperature on the ultrastructure and microtubule organization of interphase and dividing cells of the seagrass Cymodocea nodosa.Protoplasma · 2016Article
- Organization of microtubule assemblies in Dictyostelium syncytia depends on the microtubule crosslinker, Ase1.Cellular and molecular life sciences : CMLS · 2016Article
- Modelling the role of catastrophe, crossover and katanin-mediated severing in the self-organisation of plant cortical microtubules.IET systems biology · 2015Article
- MAPs: cellular navigators for microtubule array orientations in Arabidopsis.Plant cell reports · 2014Review
- Single-molecule analysis of the microtubule cross-linking protein MAP65-1 reveals a molecular mechanism for contact-angle-dependent microtubule bundling.Biophysical journal · 2012Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In plants, cortical microtubules (MTs) occur in characteristically parallel groups maintained up to one microtubule diameter apart by fine filamentous cross-bridges. However, none of the plant microtubule-associated proteins (MAPs) so far purified accounts for the observed separation between MTs in cells. We previously isolated from carrot cytoskeletons a MAP fraction including 120- and 65-kDa MAPs and have now separated the 65-kDa carrot MAP by sucrose density centrifugation. MAP65 does not induce tubulin polymerization but induces the formation of bundles of parallel MTs in a nucleotide-insensitive manner. The bundling effect is inhibited by porcine MAP2, but, unlike MAP2, MAP65 is heat-labile. In the electron microscope, MAP65 appears as filamentous cross-bridges, maintaining an intermicrotubule spacing of 25-30 nm. Microdensitometer-computer correlation analysis reveals that the cross-bridges are regularly spaced, showing a regular axial spacing that is compatible with a symmetrical helical superlattice for 13 protofilament MTs. Because MAP65 maintains in vitro the inter-MT spacing observed in plants and is shown to decorate cortical MTs, it is proposed that this MAP is important for the organization of the cortical array in vivo.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.