ArticleProceedings of the National Academy of Sciences of the United States of America1999
Intracellular localization of P1 ParB protein depends on ParA and parS.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 121 citations in OpenAlex.
- Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The ParB-CTP cycle activates phase separation in bacterial DNA segregation.Nucleic acids research · 2025Article
- The polyadenylase PAPI is required for virulence plasmid maintenance in pathogenic bacteria.PLoS pathogens · 2025Article
- VirB, a key transcriptional regulator ofmBio · 2023Article
- Septum site placement inMicrobiology (Reading, England) · 2023Article
- Article
- VirB, a key transcriptional regulator ofbioRxiv : the preprint server for biology · 2023Article
- Dissection of the ATPase active site of McdA reveals the sequential steps essential for carboxysome distribution.Molecular biology of the cell · 2021Article
- The McdAB system positions α-carboxysomes in proteobacteria.Molecular microbiology · 2021Article
- VirB, a key transcriptional regulator of virulence plasmid genes inJournal of bacteriology · 2021Article
- Physical Modeling of a Sliding Clamp Mechanism for the Spreading of ParB at Short Genomic Distance from Bacterial Centromere Sites.iScience · 2020Article
- Bacterial chromosome segregation by the ParABS system.Open biology · 2020Review
- Article
- Addressing the role of centromere sites in activation of ParB proteins for partition complex assembly.PloS one · 2020Article
- Plasmid Localization and Partition inEcoSal Plus · 2019Review
- SbnI is a free serine kinase that generatesThe Journal of biological chemistry · 2018Article
- Article
- Function, expression, specificity, diversity and incompatibility of actinobacteriophage parABS systems.Molecular microbiology · 2016Article
- Insights into ParB spreading from the complex structure of Spo0J and parS.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The P1 partition system promotes faithful plasmid segregation during the Escherichia coli cell cycle. This system consists of two proteins, ParA and ParB, that act on a plasmid site called parS. By immunofluorescence microscopy, we observed that ParB localizes to discrete foci that are most often located close to the one-quarter and three-quarters positions of cell length. The visualization of ParB foci depended completely on the presence of parS, although their visualization was independent of the chromosomal context of parS (in P1 or the bacterial chromosome). In integration host factor-defective mutants, in which ParB binding to parS is weakened, only a fraction of the total pool of ParB had converged into foci. Taken together, these results indicate that parS recruits a pool of ParB into foci and that the resulting ParB-parS complexes serve as substrates for the segregation reaction. In the absence of ParA, the position of ParB foci in cells is perturbed, indicating that at least one of the roles of ParA is to direct ParB-parS complexes to the proper one-quarter positions from a cell pole. Finally, inhibition of cell division did not inhibit localization of ParB foci in cells, indicating that the positioning signals in the E. coli host that are needed for P1 partition do not depend on early division events.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.