ArticleProceedings of the National Academy of Sciences of the United States of America1999
Genomic structure and ecdysone regulation of the prophenoloxidase 1 gene in the malaria vector Anopheles gambiae.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 100 citations in OpenAlex.
- Higher temperature accelerates the aging-dependent weakening of the melanization immune response in mosquitoes.PLoS pathogens · 2024Article
- Genome-Wide Identification and Expression Pattern of Cytochrome P450 Genes in the Social AphidInsects · 2023Article
- Ecdysone signaling mediates the trade-off between immunity and reproduction via suppression of amyloids in the mosquito Aedes aegypti.PLoS pathogens · 2022Article
- iTRAQ Proteomic Analysis of Interactions Between 20E and Phospholipase C inFrontiers in physiology · 2022Article
- Hemocyte-targeted gene expression in the female malaria mosquito using the hemolectin promoter from Drosophila.Insect biochemistry and molecular biology · 2020Article
- A mosquito juvenile hormone binding protein (mJHBP) regulates the activation of innate immune defenses and hemocyte development.PLoS pathogens · 2020Article
- Article
- Anopheline Reproductive Biology: Impacts on Vectorial Capacity and Potential Avenues for Malaria Control.Cold Spring Harbor perspectives in medicine · 2017Review
- Anopheles gambiae larvae mount stronger immune responses against bacterial infection than adults: evidence of adaptive decoupling in mosquitoes.Parasites & vectors · 2017Article
- Article
- Article
- Anopheles gambiae blood feeding initiates an anticipatory defense response to Plasmodium berghei.Journal of innate immunity · 2015Article
- Sexual transfer of the steroid hormone 20E induces the postmating switch in Anopheles gambiae.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Article
- Comparative genomics allows the discovery of cis-regulatory elements in mosquitoes.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Hormonal regulation of the humoral innate immune response in Drosophila melanogaster.The Journal of experimental biology · 2008Article
- Transcriptional analysis of an immune-responsive serine protease from Indian malarial vector, Anopheles culicifacies.BMC molecular biology · 2007Article
- Gene structure and expression profile of Manduca sexta prophenoloxidase-activating proteinase-3 (PAP-3), an immune protein containing two clip domains.Insect molecular biology · 2005Article
- Manduca sexta prophenoloxidase activating proteinase-1 (PAP-1) gene: organization, expression, and regulation by immune and hormonal signals.Insect biochemistry and molecular biology · 2005Article
- Decreased survival of mosquito cells after stable transfection with a Drosophila ecdysteroid response element: possible involvement of a 40 kDa DNA binding protein.Journal of insect science (Online) · 2002Article
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Prophenoloxidase, a melanin-synthesizing enzyme, is considered to be an important arthropod immune protein. In mosquitoes, prophenoloxidase has been shown to be involved in refractory mechanisms against malaria parasites. In our study we used Anopheles gambiae, the most important human malaria vector, to characterize the first arthropod prophenoloxidase gene at the genomic level. The complete nucleotide sequence, including the immediate 5' flanking sequence (-855 bp) of the prophenoloxidase 1 gene, was determined. The gene spans 10 kb and is composed of five exons and four introns coding for a 2.5-kb mRNA. In the 5' flanking sequence, we found several putative regulatory motifs, two of which were identified as ecdysteroid regulatory elements. Electrophoretic mobility gel-shift assays and supershift assays demonstrated that the Aedes aegypti ecdysone receptor/Ultraspiracle nuclear receptor complex, and, seemingly, the endogenous Anopheles gambiae nuclear receptor complex, was able to bind one of the ecdysteroid response elements. Furthermore, 20-hydroxyecdysone stimulation was shown to up-regulate the transcription of the prophenoloxidase 1 gene in an A. gambiae cell line.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.