ArticleProceedings of the National Academy of Sciences of the United States of America1999
Identification of a cytochrome b-type NAD(P)H oxidoreductase ubiquitously expressed in human cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 97 citations in OpenAlex.
- A functional map of phosphoprotein phosphatase regulation identifies an evolutionarily conserved reductase for the catalytic metal ions.Nature communications · 2026Article
- Cytochrome bFree radical biology & medicine · 2026Article
- Unique electron transfer system of cytochrome P450 monooxygenase includes a mechanism of fatty acid β-oxidation.Communications biology · 2025Article
- CytochromebioRxiv : the preprint server for biology · 2025Article
- Article
- Mild COVID-19 imprints a long-term inflammatory eicosanoid- and chemokine memory in monocyte-derived macrophages.Mucosal immunology · 2022Article
- Intracellular imaging of metmyoglobin and oxygen using new dual purpose probe EYFP-Myoglobin-mCherry.Journal of biophotonics · 2022Article
- Article
- Crystal structures of the naturally fused CS and cytochrome bActa crystallographica. Section D, Structural biology · 2019Article
- NCB5OR Deficiency in the Cerebellum and Midbrain Leads to Dehydration and Alterations in Thirst Response, Fasted Feeding Behavior, and Voluntary Exercise in Mice.Cerebellum (London, England) · 2018Article
- Loss of NCB5OR in the cerebellum disturbs iron pathways, potentiates behavioral abnormalities, and exacerbates harmaline-induced tremor in mice.Metabolic brain disease · 2016Article
- Practicing biochemistry without a license.The Journal of biological chemistry · 2013Article
- NAD(P)H cytochrome b5 oxidoreductase deficiency in Leishmania major results in impaired linoleate synthesis followed by increased oxidative stress and cell death.The Journal of biological chemistry · 2012Article
- Pulmonary endothelial cell NOX.American journal of respiratory cell and molecular biology · 2012Review
- OnpA, an unusual flavin-dependent monooxygenase containing a cytochrome b(5) domain.Journal of bacteriology · 2012Article
- Beta-Cell Injury in Ncb5or-null Mice is Exacerbated by Consumption of a High-Fat Diet.European journal of lipid science and technology : EJLST · 2012Article
- Another look at the interaction between mitochondrial cytochrome c and flavocytochrome b (2).European biophysics journal : EBJ · 2011Review
- Development of diabetes in lean Ncb5or-null mice is associated with manifestations of endoplasmic reticulum and oxidative stress in beta cells.Biochimica et biophysica acta · 2011Article
- Ncb5or deficiency increases fatty acid catabolism and oxidative stress.The Journal of biological chemistry · 2011Article
- Study of the individual cytochrome b5 and cytochrome b5 reductase domains of Ncb5or reveals a unique heme pocket and a possible role of the CS domain.The Journal of biological chemistry · 2010Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Cytochrome b-type NAD(P)H oxidoreductases are involved in many physiological processes, including iron uptake in yeast, the respiratory burst, and perhaps oxygen sensing in mammals. We have identified a cytosolic cytochrome b-type NAD(P)H oxidoreductase in mammals, a flavohemoprotein (b5+b5R) containing cytochrome b5 (b5) and b5 reductase (b5R) domains. A genetic approach, using BLAST searches against DBEST for FAD-, NAD(P)H-binding sequences followed by reverse transcription-PCR, was used to clone the complete cDNA sequence of human b5+b5R from the hepatoma cell line Hep 3B. Compared with the classical single-domain b5 and b5R proteins localized on endoplasmic reticulum membrane, b5+b5R also has binding motifs for heme, FAD, and NAD(P)H prosthetic groups but no membrane anchor. The human b5+b5R transcript was expressed at similar levels in all tissues and cell lines that were tested. The two functional domains b5* and b5R* are linked by an approximately 100-aa-long hinge bearing no sequence homology to any known proteins. When human b5+b5R was expressed as c-myc adduct in COS-7 cells, confocal microscopy revealed a cytosolic localization at the perinuclear space. The recombinant b5+b5R protein can be reduced by NAD(P)H, generating spectrum typical of reduced cytochrome b with alpha, beta, and Soret peaks at 557, 527, and 425 nm, respectively. Human b5+b5R flavohemoprotein is a NAD(P)H oxidoreductase, demonstrated by superoxide production in the presence of air and excess NAD(P)H and by cytochrome c reduction in vitro. The properties of this protein make it a plausible candidate oxygen sensor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.