ArticleNucleic acids research2000
UPA, a universal protein array system for quantitative detection of protein-protein, protein-DNA, protein-RNA and protein-ligand interactions.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 243 citations in OpenAlex.
- A simple and rapid pipeline for identification of receptor-binding sites on the surface proteins of pathogens.Scientific reports · 2020Article
- Article
- A New Method for Immobilization of His-Tagged Proteins with the Application of Low-Frequency AC Electric Field.Sensors (Basel, Switzerland) · 2018Article
- Computational analysis of protein interaction networks for infectious diseases.Briefings in bioinformatics · 2016Review
- Optimized lysis buffer reagents for solubilization and preservation of proteins from cells and tissues.Drug delivery and translational research · 2013Article
- SMETANA: accurate and scalable algorithm for probabilistic alignment of large-scale biological networks.PloS one · 2013Article
- Functional protein microarray as molecular decathlete: a versatile player in clinical proteomics.Proteomics. Clinical applications · 2012Review
- Development of an Interaction Assay between Single-Stranded Nucleic Acids Trapped with Silica Particles and Fluorescent Compounds.Journal of functional biomaterials · 2012Article
- Article
- Serologic laboratory findings in malignancy.Rheumatic diseases clinics of North America · 2011Review
- Extensive characterization of NF-κB binding uncovers non-canonical motifs and advances the interpretation of genetic functional traits.Genome biology · 2011Article
- Article
- A semi-nonparametric mixture model for selecting functionally consistent proteins.BMC bioinformatics · 2010Article
- Identifying protein interactions with metal-modified DNA using microarray technology.Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry · 2009Article
- Article
- Proteomics Discovery of Disease Biomarkers.Biomarker insights · 2008Article
- A proteome chip approach reveals new DNA damage recognition activities in Escherichia coli.Nature methods · 2008Article
- Validation and quality control of protein microarray-based analytical methods.Molecular biotechnology · 2008Review
- High-throughput three-dimensional gel electrophoresis for versatile utilities: a stacked slice-gel system for separation and reactions (4SR).Genomics, proteomics & bioinformatics · 2006Article
- Distinctive serum protein profiles involving abundant proteins in lung cancer patients based upon antibody microarray analysis.BMC cancer · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
1 author at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein-protein interactions have been widely used to study gene expression pathways and may be considered as a new approach to drug discovery. Here I report the development of a universal protein array (UPA) system that provides a sensitive, quantitative, multi-purpose, effective and easy technology to determine not only specific protein-protein interactions, but also specific interactions of proteins with DNA, RNA, ligands and other small chemicals. (i) Since purified proteins are used, the results can be easily interpreted. (ii) UPA can be used multiple times for different targets, making it economically affordable for most laboratories, hospitals and biotechnology companies. (iii) Unlike DNA chips or DNA microarrays, no additional instrumentation is required. (iv) Since the UPA uses active proteins (without denaturation and renaturation), it is more sensitive compared with most existing methods. (v) Because the UPA can analyze hundreds (even thousands on a protein microarray) of proteins in a single experiment, it is a very effective method to screen proteins as drug targets in cancer and other human diseases.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.