ArticleNucleic acids research2000
Transcript quantitation in total yeast cellular RNA using kinetic PCR.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- A Memoir of Inventing Real-Time PCR and Developing the ABI 7700.International journal of molecular sciences · 2026Article
- Article
- In vivo expression of Helicobacter pylori virulence genes in patients with gastritis, ulcer, and gastric cancer.Infection and immunity · 2012Article
- Conserved transcriptional unit organization of the cag pathogenicity island among Helicobacter pylori strains.Frontiers in cellular and infection microbiology · 2012Article
- Assessing the performance capabilities of LRE-based assays for absolute quantitative real-time PCR.PloS one · 2010Article
- Conditional inhibition of autophagy genes in adult Drosophila impairs immunity without compromising longevity.Experimental gerontology · 2009Article
- Discarding duplicate ditags in LongSAGE analysis may introduce significant error.BMC bioinformatics · 2007Article
- Low abundance of telomerase in yeast: implications for telomerase haploinsufficiency.RNA (New York, N.Y.) · 2006Article
- Evaluation of control transcripts in real-time RT-PCR expression analysis during maritime pine embryogenesis.Planta · 2005Article
- Sigmoidal curve-fitting redefines quantitative real-time PCR with the prospective of developing automated high-throughput applications.Nucleic acids research · 2004Article
- Modification of Helicobacter pylori outer membrane protein expression during experimental infection of rhesus macaques.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Expression profiles frame the promoter specificity dilemma of the ETS family of transcription factors.Nucleic acids research · 2004Article
- Mathematics of quantitative kinetic PCR and the application of standard curves.Nucleic acids research · 2003Article
- Quantitative assay for measuring the Taura syndrome virus and yellow head virus load in shrimp by real-time RT-PCR using SYBR Green chemistry.Journal of virological methods · 2002Article
- High-throughput SNP allele-frequency determination in pooled DNA samples by kinetic PCR.Genome research · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and SAGE technologies for certain applications. The reproducibility, sensitivity and accuracy of the kRT-PCR were assessed for yeast transcripts previously quantitated by a variety of methods including SAGE analysis. Changes in transcript levels between different genetic or physiological cell states were reproducibly quantitated with an accuracy of +/-20%. The assay was sufficiently sensitive to quantitate yeast transcripts over a range of more than five orders of magnitude, including low abundance transcripts encoding cell cycle and transcriptional regulators.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.