ArticleNucleic acids research2000
Histidylated oligolysines increase the transmembrane passage and the biological activity of antisense oligonucleotides.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 63 citations in OpenAlex.
- Evaluation of Histidine-Octamer-Modified Hyaluronic Acid as a Cytosolic Drug Delivery Material via CD44-Mediated Cellular Uptake and Endosomal Escape.Pharmaceutics · 2026Article
- The Multifaceted Histidine-Based Carriers for Nucleic Acid Delivery: Advances and Challenges.Pharmaceutics · 2020Review
- Self-Crosslinking Lipopeptide/DNA/PEGylated Particles: A New Platform for DNA Vaccination Designed for Assembly in Aqueous Solution.Molecular therapy. Nucleic acids · 2018Article
- Solid-phase supported design of carriers for therapeutic nucleic acid delivery.Bioscience reports · 2017Review
- Tissue-specific Calibration of Real-time PCR Facilitates Absolute Quantification of Plasmid DNA in Biodistribution Studies.Molecular therapy. Nucleic acids · 2016Article
- Bioreducible polycations as shuttles for therapeutic nucleic acid and protein transfection.Antioxidants & redox signaling · 2014Review
- Article
- Influence of histidine incorporation on buffer capacity and gene transfection efficiency of HPMA-co-oligolysine brush polymers.Biomacromolecules · 2013Article
- Melittin derived peptides for nanoparticle based siRNA transfection.Biomaterials · 2013Article
- An influenza virus-inspired polymer system for the timed release of siRNA.Nature communications · 2013Article
- Buffering capacity and size of siRNA polyplexes influence cytokine levels.Molecular therapy : the journal of the American Society of Gene Therapy · 2012Article
- Nanoparticles: a promising modality in the treatment of sarcomas.Pharmaceutical research · 2011Review
- Missing pieces in understanding the intracellular trafficking of polycation/DNA complexes.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
- Chemical vectors for gene delivery: a current review on polymers, peptides and lipids containing histidine or imidazole as nucleic acids carriers.British journal of pharmacology · 2009Review
- Peptide-guided gene delivery.The AAPS journal · 2007Review
- Design and development of polymers for gene delivery.Nature reviews. Drug discovery · 2005Review
- Modified branched peptides with a histidine-rich tail enhance in vitro gene transfection.Nucleic acids research · 2005Article
- Enhanced transfection efficiency of a systemically delivered tumor-targeting immunolipoplex by inclusion of a pH-sensitive histidylated oligolysine peptide.Nucleic acids research · 2004Article
- Intracellular processing of poly(ethylene imine)/ribozyme complexes can be observed in living cells by using confocal laser scanning microscopy and inhibitor experiments.Pharmaceutical research · 2002Article
- Inhibition of HIV-1 in cell culture by oligonucleotide-loaded nanoparticles.Pharmaceutical research · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have designed histidylated oligolysines which increase the uptake, the cytosolic delivery and the nuclear accumulation of antisense oligonucleotides (ODN). Flow cytometry analysis showed a 10-fold enhancement of the ODN uptake in the presence of histidylated oligolysines. The intracellular localizations of fluorescein-labeled ODN and of rhodamine-labeled histidylated oligolysines were investigated by confocal microscopy. Histidylated oligolysines favor the cyto-solic delivery of ODN from endosomes and increase their nuclear accumulation. In contrast, in their absence fluorescent ODN were not observed inside the nucleus but were distributed overwhelmingly within the vesicles in the cytosol. In addition, histidylated oligolysines yielded a more than 20-fold enhancement of the biological activity of antisense ODN towards the inhibition of transient as well as constitutive gene expression. Prevention of endosome lumen acidification using bafilomycin A(1)abolished the effect of histidylated oligolysines, suggesting that protonation of the histidyl residues was involved in the transmembrane passage of ODN.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.