ArticleNucleic acids research2000
In vitro selection and characterization of a highly efficient Zn(II)-dependent RNA-cleaving deoxyribozyme.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 122 papers.
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Who cites it
122 citing papers in PubMed, 454 citations in OpenAlex.
- Fully Reversible Regulation of 8-17 DNAzyme Activity Using Nucleoside-Based Diarylethenes.Chemistry (Weinheim an der Bergstrasse, Germany) · 2026Article
- DNAzyme-Based Nanostructures for Dynamic Cell Regulation and Sensing.ChemistryOpen · 2026Review
- A minimal RNA-cleaving DNAzyme and its catalytic mechanism.Nucleic acids research · 2026Article
- Cooperative Mismatch Discrimination by PNA and DNAzyme Enables High-Fidelity Cleavage of Plasmid DNA.Biochemistry · 2025Article
- Decoding Potassium Homeostasis in Cancer Metastasis and Drug Resistance: Insights from a Highly Selective DNAzyme-Based Intracellular KJournal of the American Chemical Society · 2025Article
- A Modular Engineered DNA Nanodevice for Precise Profiling of Telomerase RNA Location and Activity.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Technologies for Targeted RNA Degradation and Induced RNA Decay.Chemical reviews · 2024Review
- Recent Advances and Prospects of Nucleic Acid Therapeutics for Anti-Cancer Therapy.Molecules (Basel, Switzerland) · 2024Review
- Design of a Membrane-Anchored DNAzyme-Based Molecular Machine for Enhanced Cancer Therapy by Customized Cascade Regulation.ACS pharmacology & translational science · 2024Article
- The Programmable Catalytic Core of 8-17 DNAzymes.Molecules (Basel, Switzerland) · 2024Article
- Detection of intracellular microRNA-21 for cancer diagnosis by a nanosystem containing a ZnO@polydopamine and DNAzyme probe.RSC advances · 2024Article
- Rectifying artificial nanochannels with multiple interconvertible permeability states.Nature communications · 2024Article
- Improving the Sensitivity of a Mn(II)-Specific DNAzyme for Cellular Imaging Sensor through Sequence Mutations.Analytical chemistry · 2024Article
- Ratiometric Detection of ZnChemistry (Basel, Switzerland) · 2023Article
- Review
- Simultaneous FeScience advances · 2023Article
- Construction and Application of DNAzyme-based Nanodevices.Chemical research in Chinese universities · 2023Review
- Combination Cancer Treatment: Using Engineered DNAzyme Molecular Machines for Dynamic Inter- and Intracellular Regulation.Angewandte Chemie (International ed. in English) · 2022Article
- DNAzyme-Based Lithium-Selective Imaging Reveals Higher Lithium Accumulation in Bipolar Disorder Patient-Derived Neurons.ACS central science · 2021Article
- RNA-Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA.Angewandte Chemie (International ed. in English) · 2021Article
62 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors at 1 institution in 1 country.
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Abstract
A group of highly efficient Zn(II)-dependent RNA-cleaving deoxyribozymes has been obtained through in vitro selection. They share a common motif with the '8-17' deoxyribozyme isolated under different conditions, including different design of the random pool and metal ion cofactor. We found that this commonly selected motif can efficiently cleave both RNA and DNA/RNA chimeric substrates. It can cleave any substrate containing rNG (where rN is any ribo-nucleotide base and G can be either ribo- or deoxy-ribo-G). The pH profile and reaction products of this deoxyribozyme are similar to those reported for hammerhead ribozyme. This deoxyribozyme has higher activity in the presence of transition metal ions compared to alkaline earth metal ions. At saturating concentrations of Zn(2+), the cleavage rate is 1.35 min(-1)at pH 6.0; based on pH profile this rate is estimated to be at least approximately 30 times faster at pH 7.5, where most assays of Mg(2+)-dependent DNA and RNA enzymes are carried out. This work represents a comprehensive characterization of a nucleic acid-based endonuclease that prefers transition metal ions to alkaline earth metal ions. The results demonstrate that nucleic acid enzymes are capable of binding transition metal ions such as Zn(2+)with high affinity, and the resulting enzymes are more efficient at RNA cleavage than most Mg(2+)-dependent nucleic acid enzymes under similar conditions.
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