ArticleNucleic acids research2000
Nucleoplasmin-mediated chromatin remodelling is required for Xenopus sperm nuclei to become licensed for DNA replication.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 43 citations in OpenAlex.
- RNAs coordinate nuclear envelope assembly and DNA replication through ELYS recruitment to chromatin.Nature communications · 2017Article
- The High-Affinity Interaction between ORC and DNA that Is Required for Replication Licensing Is Inhibited by 2-Arylquinolin-4-Amines.Cell chemical biology · 2017Article
- GON4L Drives Cancer Growth through a YY1-Androgen Receptor-CD24 Axis.Cancer research · 2016Article
- Presence of the Paternal Pronucleus Assists Embryo in Overcoming Cycloheximide Induced Abnormalities in Zygotic Mitosis.Journal of cellular biochemistry · 2016Article
- Depletion of Uhrf1 inhibits chromosomal DNA replication in Xenopus egg extracts.Nucleic acids research · 2013Article
- MCM2-7 form double hexamers at licensed origins in Xenopus egg extract.The Journal of biological chemistry · 2011Article
- Functional cooperation between FACT and MCM is coordinated with cell cycle and differential complex formation.Journal of biomedical science · 2010Article
- A strong nucleotypic effect on the cell cycle regardless of ploidy level.Annals of botany · 2008Article
- Identification of novel human Cdt1-binding proteins by a proteomics approach: proteolytic regulation by APC/CCdh1.Molecular biology of the cell · 2008Article
- Cdt1 downregulation by proteolysis and geminin inhibition prevents DNA re-replication in Xenopus.The EMBO journal · 2005Article
- The role of Cdc6 in ensuring complete genome licensing and S phase checkpoint activation.The Journal of cell biology · 2004Article
- Non-proteolytic inactivation of geminin requires CDK-dependent ubiquitination.Nature cell biology · 2004Article
- Cell cycle-dependent regulation of the association between origin recognition proteins and somatic cell chromatin.The EMBO journal · 2002Article
- The Drosophila nuclear lamina protein YA binds to DNA and histone H2B with four domains.Molecular biology of the cell · 2002Article
- Repression of origin assembly in metaphase depends on inhibition of RLF-B/Cdt1 by geminin.Nature cell biology · 2001Article
- Replication origins in Xenopus egg extract Are 5-15 kilobases apart and are activated in clusters that fire at different times.The Journal of cell biology · 2001Article
- Reconstitution of licensed replication origins on Xenopus sperm nuclei using purified proteins.BMC biochemistry · 2001Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
During late mitosis and early G(1), a series of proteins are assembled onto replication origins, resulting in them becoming 'licensed' for replication in the subsequent S phase. Four factors have so far been identified that are required for chromatin to become functionally licensed: ORC (the origin recognition complex) and Cdc6, plus the two components of the replication licensing system RLF-M and RLF-B. Here we describe the first steps of a systematic fractionation of Xenopus egg extracts to identify all the components necessary for the assembly of licensed replication origins on Xenopus sperm nuclei (the physiological DNA substrate in this system). We have purified a new activity essential for this reaction, and have shown that it is nucleoplasmin, a previously known chromatin remodelling protein. Nucleoplasmin decondenses the sperm chromatin by removing protamines, and is required at the earliest known step in origin assembly to allow ORC to bind to the DNA. Sperm nuclei can be licensed by a combination of nucleoplasmin, RLF-M and a partially purified fraction that contains ORC, Cdc6 and RLF-B. This suggests that we are likely to have identified most of the proteins required for this assembly reaction.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.