ArticleNucleic acids research2000
An exonic splicing silencer in the testes-specific DNA ligase III beta exon.
Article in Nucleic acids research, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed, 24 citations in OpenAlex.
- Multiple tandem splicing silencer elements suppress aberrant splicing within the long exon 26 of the human Apolipoprotein B gene.BMC molecular biology · 2013Article
- Identification of splicing silencers and enhancers in sense Alus: a role for pseudoacceptors in splice site repression.Molecular and cellular biology · 2005Article
- Pseudoexon activation as a novel mechanism for disease resulting in atypical growth-hormone insensitivity.American journal of human genetics · 2001Article
- Caspase-2 pre-mRNA alternative splicing: Identification of an intronic element containing a decoy 3' acceptor site.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Mitochondrial DNA ligase III function is independent of Xrcc1.Nucleic acids research · 2000Article
- Control of hnRNP A1 alternative splicing: an intron element represses use of the common 3' splice site.Molecular and cellular biology · 2000Article
- Regulation of alternative RNA splicing by exon definition and exon sequences in viral and mammalian gene expression.Journal of biomedical scienceReview
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Alternative pre-mRNA splicing of two terminal exons (alpha and beta) regulates the expression of the human DNA ligase III gene. In most tissues, the alpha exon is expressed. In testes and during spermatogenesis, the beta exon is used instead. The alpha exon encodes the interaction domain with a scaffold DNA repair protein, XRCC1, while the beta exon-encoded C-terminal does not. Sequence elements regulating the alternative splicing pattern were mapped by in vitro splicing assays in HeLa nuclear extracts. Deletion of a region beginning in the beta exon and extending into the downstream intron derepressed splicing to the beta exon. Two silencing elements were found within this 101 nt region: a 16 nt exonic splicing silencer immediately upstream of the beta exon polyadenylation signal and a 45 nt intronic splicing silencer. The exonic splicing silencer inhibited splicing, even when the poly-adenylation signal was deleted or replaced by a 5' splice site. This element also enhanced polyadenylation under conditions unfavourable to splicing. The splicing silencer partially inhibited assembly of spliceo-somal complexes and functioned in an adenoviral pre-mRNA context. Silencing of splicing by the element was associated with cross-linking of a 37 kDa protein to the RNA substrate. The element exerts opposite functions in splicing and polyadenylation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.