ArticleRNA (New York, N.Y.)1999
In vitro selection identifies key determinants for loop-loop interactions: RNA aptamers selective for the TAR RNA element of HIV-1.
Article in RNA (New York, N.Y.), 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Are we there yet with XNA aptamers?RSC advances · 2026Review
- High-Throughput Sequencing and SELEX-Based Protocol for Selecting Aptamers Against Potato Spindle Tuber Viroid.International journal of molecular sciences · 2026Article
- The Use of Xenonucleic Acids Significantly Reduces the In Vivo Drift of Electrochemical Aptamer-Based Sensors.Angewandte Chemie (International ed. in English) · 2024Article
- Regulation of Gene Expression Through Effector-dependent Conformational Switching by Cobalamin Riboswitches.Journal of molecular biology · 2022Review
- Unraveling the structure and biological functions of RNA triple helices.Wiley interdisciplinary reviews. RNA · 2020Review
- Human Immunodeficiency Virus-Associated Exosomes Promote Kaposi's Sarcoma-Associated Herpesvirus Infection via the Epidermal Growth Factor Receptor.Journal of virology · 2020Article
- In vitro selection of l-DNA aptamers that bind a structured d-RNA molecule.Nucleic acids research · 2020Article
- Exosomes derived from HIV-1-infected cells promote growth and progression of cancer via HIV TAR RNA.Nature communications · 2018Article
- Triggering nucleic acid nanostructure assembly by conditional kissing interactions.Nucleic acids research · 2018Article
- On the conformational stability of the smallest RNA kissing complexes maintained through two G·C base pairs.Biochemical and biophysical research communications · 2017Article
- Aptamers against pathogenic microorganisms.Critical reviews in microbiology · 2016Review
- A combinatorial approach to the repertoire of RNA kissing motifs; towards multiplex detection by switching hairpin aptamers.Nucleic acids research · 2016Article
- Specific Inhibition of MicroRNA Processing Using L-RNA Aptamers.Journal of the American Chemical Society · 2015Article
- Fitness Landscapes of Functional RNAs.Life (Basel, Switzerland) · 2015Review
- High-affinity RNA Aptamers Against the HIV-1 Protease Inhibit Both In Vitro Protease Activity and Late Events of Viral Replication.Molecular therapy. Nucleic acids · 2015Article
- Efficient HIV-1 inhibition by a 16 nt-long RNA aptamer designed by combining in vitro selection and in silico optimisation strategies.Scientific reports · 2014Article
- Aptamer-based therapeutics: new approaches to combat human viral diseases.Pharmaceuticals (Basel, Switzerland) · 2013Article
- Unmasking the information encoded as structural motifs of viral RNA genomes: a potential antiviral target.Reviews in medical virology · 2013Review
- Binding of a structured D-RNA molecule by an L-RNA aptamer.Journal of the American Chemical Society · 2013Article
- Inhibition of HIV Replication by Cyclic and Hairpin PNAs Targeting the HIV-1 TAR RNA Loop.Journal of nucleic acids · 2012Article
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2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We selected RNA aptamers specific for the trans-activation responsive (TAR) RNA, a stem-loop structure crucial for the transcription of the integrated genome of the human immunodeficiency virus. Most of the selected sequences could be folded as imperfect hairpins and displayed a 5'-GUCCCAGA-3' consensus motif constituting the apical loop. The six central bases of this consensus sequence are complementary to the entire TAR loop, leading to the formation of TAR RNA-aptamer "kissing" complexes. The consensus G and A residues closing the aptamer loop contributed to the high affinity (Kd = 30 nM at 23 degrees C) of the aptamers for the TAR RNA. This G A pair was shown to be crucial for binding to TAR at a low magnesium concentration. The selection also identified 5'-PuPy and 5'-PyPu base pairs at alpha and beta positions of the stem, next to the loop, respectively. This strategy offered a way to identify key determinants of loop-loop interactions and to generate high affinity ligands of TAR RNA structure.
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