ArticleInfection and immunity2000
Intracellular and extracellular cytokine production by human mixed mononuclear cells in response to group B streptococci.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 58 citations in OpenAlex.
- Moderate- and high-intensity exhaustive exercise in the heat induce a similar increase in monocyte Hsp72.Cell stress & chaperones · 2015Trial
- IL-1β promotes the differentiation of polyfunctional human CCR6+CXCR3+ Th1/17 cells that are specific for pathogenic and commensal microbes.Journal of immunology (Baltimore, Md. : 1950) · 2014Trial
- A Comparison of Neutrophil Secretion of Cytokines and NET Formation in Response to Lipopolysaccharide and Group BJournal of inflammation research · 2026Article
- Wnt signaling regulates chemokine production and cell migration of circulating human monocytes.Cell communication and signaling : CCS · 2024Article
- Association Between Albumin Levels and Neonatal Acute Respiratory Distress Syndrome in Newborn Pneumoniae.Journal of inflammation research · 2024Article
- A nomogram for predicting neonatal acute respiratory distress syndrome in patients with neonatal pneumonia after 34 weeks of gestation.Frontiers in pediatrics · 2024Article
- The causal role of intestinal microbiome in development of pre-eclampsia.Functional & integrative genomics · 2023Article
- Serum/glucocorticoid-inducible kinase 1 deficiency induces NLRP3 inflammasome activation and autoinflammation of macrophages in a murine endolymphatic hydrops model.Nature communications · 2023Article
- Hypoxia-Inducible Factor 1 Alpha Is Dispensable for Host Defense of Group B Streptococcus Colonization and Infection.Journal of innate immunity · 2021Article
- Article
- Pneumonia.Seminars in fetal & neonatal medicine · 2017Review
- Cold stress aggravates inflammatory responses in an LPS-induced mouse model of acute lung injury.International journal of biometeorology · 2016Article
- Group B Streptococcus Induces a Robust IFN-γ Response by CD4(+) T Cells in an In Vitro and In Vivo Model.Journal of immunology research · 2016Article
- Group B streptococcal colonization and the risk of pre-eclampsia.Epidemiology and infection · 2013Article
- The β-hemolysin and intracellular survival of Streptococcus agalactiae in human macrophages.PloS one · 2013Article
- Suppressive effects of sivelestat on interleukin 8 and TNF-α production from LPS-stimulated granulocytes in whole blood culture.Journal of anesthesia · 2010Article
- Multidimensional analysis of the frequencies and rates of cytokine secretion from single cells by quantitative microengraving.Lab on a chip · 2010Article
- Subcompartments of the macrophage recycling endosome direct the differential secretion of IL-6 and TNFalpha.The Journal of cell biology · 2007Article
- Adherence to, invasion by, and cytokine production in response to serotype VIII group B Streptococci.Infection and immunity · 2004Article
- Critical role of the complement system in group B streptococcus-induced tumor necrosis factor alpha release.Infection and immunity · 2003Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Group B streptococci (GBS) are a major cause of severe infection in newborns, pregnant females, and other immunocompromised hosts. Infection often includes septicemia, shock, pneumonia, and respiratory failure. In previous studies, we have reported that GBS induce marked production of tumor necrosis factor alpha (TNF-alpha) by human mononuclear cells. The present study was designed to measure the production of TNF-alpha as well as additional cytokines, including interleukin 1beta (IL-1beta), IL-6, IL-8, IL-12, and gamma interferon (IFN-gamma) but also to determine from what cells and at what time point during incubation with GBS that these cytokines are produced. Mixed mononuclear cells were incubated with heat-killed GBS, media alone, or 1 microg of Escherichia coli lipopolysaccharide (LPS). Brefeldin A was added to each sample prior to staining, which prevented the export of cytokines by the Golgi apparatus. The cells were then stained with the appropriate conjugated antibodies and analyzed by using a flow cytometer. Results indicate that intracellular cytokines appear, in almost all cases, simultaneous to or before secreted proteins are detected. In contrast to the response to LPS, where TNF-alpha, IL-1beta, IL-6, and IL-8 appear almost simultaneously, the human monocyte response to GBS results in the production of TNF-alpha but delayed appearance of IL-1beta, IL-6, and IL-8. The lymphocyte response to GBS was also strikingly different from that to LPS in that both secreted IFN-gamma and IL-12 was detected, while LPS failed to induce production of these critical cytokines. This suggests an important role for TNF-alpha, IFN-gamma, and IL-12 in GBS pathogenesis and/or immunity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.