ArticleInfection and immunity2000
M-like proteins of Streptococcus dysgalactiae.
Article in Infection and immunity, 2000. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 60 citations in OpenAlex.
- Epidemiological and Clinical Features ofPathogens (Basel, Switzerland) · 2023Article
- Whole genome sequencing reveals possible host species adaptation of Streptococcus dysgalactiae.Scientific reports · 2021Article
- The Role ofMicroorganisms · 2021Review
- PRD-Containing Virulence Regulators (PCVRs) in Pathogenic Bacteria.Frontiers in cellular and infection microbiology · 2021Review
- Pathogenicity Factors in Group C and G Streptococci.Microbiology spectrum · 2019Review
- Genetics and Pathogenicity Factors of Group C and G Streptococci.Microbiology spectrum · 2019Review
- Multimerization of the Virulence-Enhancing Group A Streptococcus Transcription Factor RivR Is Required for Regulatory Activity.Journal of bacteriology · 2017Article
- Technologies for Proteome-Wide Discovery of Extracellular Host-Pathogen Interactions.Journal of immunology research · 2017Review
- Exploring the Secretomes of Microbes and Microbial Communities Using Filamentous Phage Display.Frontiers in microbiology · 2016Review
- Activator role of the pneumococcal Mga-like virulence transcriptional regulator.Journal of bacteriology · 2012Article
- Vru (Sub0144) controls expression of proven and putative virulence determinants and alters the ability of Streptococcus uberis to cause disease in dairy cattle.Microbiology (Reading, England) · 2012Article
- Virulence gene pool detected in bovine group C Streptococcus dysgalactiae subsp. dysgalactiae isolates by use of a group A S. pyogenes virulence microarray.Journal of clinical microbiology · 2011Article
- Streptococcus adherence and colonization.Microbiology and molecular biology reviews : MMBR · 2009Review
- Streptococcus iniae M-like protein contributes to virulence in fish and is a target for live attenuated vaccine development.PloS one · 2008Article
- Identification and molecular characterisation of a fibrinogen binding protein from Streptococcus iniae.BMC microbiology · 2008Article
- Phage display in the study of infectious diseases.Trends in microbiology · 2006Review
- Domains required for transcriptional activation show conservation in the mga family of virulence gene regulators.Journal of bacteriology · 2006Article
- What happened to the streptococci: overview of taxonomic and nomenclature changes.Clinical microbiology reviews · 2002Review
- Identification of novel adhesins from Group B streptococci by use of phage display reveals that C5a peptidase mediates fibronectin binding.Infection and immunity · 2002Article
- Surface-expressed mig protein protects Streptococcus dysgalactiae against phagocytosis by bovine neutrophils.Infection and immunity · 2001Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus dysgalactiae is one of the most important bacterial species isolated from bovine mastitis. To identify potential virulence factors of this species we prepared chromosomal DNA from strain 8215 and constructed a phage display library. By affinity selection of the library against fibrinogen (Fg), we isolated and characterized a gene, called demA, encoding a protein with the molecular mass of approximately 58 kDa, called DemA, displaying both plasma protein binding properties and sequence similarities with the M and M-like proteins of other streptococcal species. Purified recombinant DemA protein was found to completely inhibit Fg-binding to cells of S. dysgalactiae. A continued sequence analysis revealed that the demA gene was preceded by an open reading frame (dmgA) coding for a putative protein, called DmgA, with high similarities to the Mga proteins of Streptococcus pyogenes. By additional cloning, the corresponding dmgA and demA genes from another strain, called Epi9, were isolated and analyzed. These genes, called dmgB and demB, respectively, revealed a high degree of similarity to the corresponding genes in strain 8215. Increased binding of Fg by cells of strain Epi9, grown in an atmosphere with 10% CO(2), was correlated to an enhanced transcription of the demB gene as shown in a Northern blot. Strain 8215 did not respond to CO(2), which could be explained by a nonfunctional dmgA gene due to insertion of an insertion sequence element. Based on sequence similarities of the described proteins to Mga, M, and M-like proteins and the response to elevated level of CO(2), we suggest that the dmg and dem genes are members of a regulon similar to the described mga regulon in S. pyogenes, which encodes several virulence factors in this species.
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